Semen-coagulating protein, SVS2, in mouse seminal plasma controls sperm fertility
Semen-coagulating protein, SVS2, in mouse seminal plasma controls sperm fertility
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DOI：
10.1095/biolreprod.106.056887
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发表时间：
2007-03-01
影响因子：
3.6
通讯作者：
Yoshida, Manabu
中科院分区：
文献类型：
--
作者：
Kawano, Natsuko;Yoshida, Manabu
已知哺乳动物精浆含有去获能因子，其阻止获能，从而阻止精子的生育力。在体外进行的评估精浆或纯化的去精子因子对附睾精子生育力的抑制作用的实验中观察到了这种现象。然而，在体内的现象，去血的特点还没有。在本研究中，我们证明，精囊蛋白分泌2（SVS2），这是一个40 kDa的碱性蛋白和交配栓的主要组成部分，进入子宫和交配后与射出的精子头相互作用。精子的SVS2结合区从顶体后区向赤道段转移，精子则通过子宫迁移，最后消失在输卵管中。此外，SVS2降低了附睾精子的生育力。用SVS2处理的精子使受精卵母细胞的百分比从60%降低到10%。获能状态通过蛋白质酪氨酸磷酸化和顶体反应的综合性来评估。SVS2的功能是维持精子处于未获能状态，并将获能精子逆转为未获能状态。我们发现射精精子的生育能力与SVS2在女性生殖道中的分布有关。这些结果表明，SVS 2具有小鼠精子失能因子的作用。
Mammalian seminal plasma is known to contain a decapacitation factor(s) that prevents capacitation and thus, the fertility of sperm. This phenomenon has been observed in experiments conducted in vitro that assessed the inhibition of epididymal sperm fertility by seminal plasma or by the purified decapacitation factor. However, the phenomenon of decapacitation has not yet been characterized in vivo. In the present study, we demonstrate that seminal vesicle protein secretion 2 (SVS2), which is a 40-kDa basic protein and a major component of the copulatory plug, enters the uterus and interacts with ejaculated sperm heads after copulation. The SVS2-binding region of sperm changed from the postacrosomal region to the equatorial segment, while the sperm migrated through the uterus and finally disappeared in the oviduct. Furthermore, SVS2 reduced the fertility of epididymal sperm. The sperm treated with SVS2 decreased the percentage of fertilized oocytes from 60% to 10%. The capacitation state was assessed by protein tyrosine phosphorylation and the comprehensiveness of the acrosome reaction. SVS2 functioned to maintain sperm in the uncapacitated state and to reverse capacitated sperm to the uncapacitated state. We found that the fertility of ejaculated sperm is associated with SVS2 distribution in the female reproductive tract. These results indicate that SVS2 functions as a decapacitation factor for mouse sperm.