The β-1,3-Exoglucanase Gene exgA (exg1) of Aspergillus oryzae Is Required to Catabolize Extracellular Glucan, and Is Induced in Growth on a Solid Surface
The β-1,3-Exoglucanase Gene exgA (exg1) of Aspergillus oryzae Is Required to Catabolize Extracellular Glucan, and Is Induced in Growth on a Solid Surface
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米曲霉的 β-1,3-外切葡聚糖酶基因 exgA (exg1) 是分解代谢胞外葡聚糖所必需的，并诱导其在固体表面上生长
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
M. Machida
中科院分区：
文献类型：
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作者：
Koichi Tamano;Y. Satoh;T. Ishii;Yasunobu Terabayashi;S. Ohtaki;M. Sano;Tadashi C. Takahashi;Y. Koyama;O. Mizutani;Keietsu Abe;M. Machida
分析了黑曲霉分泌型β-1，3-外切葡聚糖酶ExgA（Exg 1）的生物学功能及其基因表达模式。构建了exgA破坏子和exgA过表达突变体，并比较了两种突变体的表型。当以β-1，3-葡聚糖为唯一碳源时，exgA过量表达突变体的菌丝生长速率和产孢效率均高于exgA破坏突变体。另一方面，当与葡萄糖一起生长时，在存在或不存在细胞壁β-葡聚糖重塑抑制剂的情况下，在它们之间没有观察到表型差异。exgA在固体表面如滤膜和洋葱内皮上的生长中被诱导表达。营养不良和菌丝附着在疏水固体表面的组合似乎是exgA表达的诱导因子。这些数据表明ExgA在β-葡聚糖利用中发挥作用，但在细胞壁β-葡聚糖重塑中没有太大作用。
The biological role of ExgA (Exg1), a secretory β-1,3-exoglucanase of Aspergillus oryzae, and the expression pattern of the exgA (exg1) gene were analyzed. The exgA disruptant and the exgA-overexpressing mutant were constructed, and phenotypes of both mutants were compared. Higher mycelial growth rate and conidiation efficiency were observed for the exgA-overexpressing mutant than for the exgA disruptant when β-1,3-glucan was supplied as sole carbon source. On the other hand, no difference in phenotype was observed between them in the presence or absence of the inhibitors of cell wall β-glucan remodeling when grown with glucose. exgA Expression was induced in growth on solid surfaces such as filter membrane and onion inner skin. A combination of poor nutrition and mycelial attachment to a hydrophobic solid surface appears to be an inducing factor for exgA expression. These data suggest that ExgA plays a role in β-glucan utilization, but is not much involved in cell wall β-glucan remodeling.
影响因子：
3.5
作者：
Beckerman, JL;Ebbole, DJ
通讯作者：
Ebbole, DJ