Role of prostaglandin in the formation of osteoclasts induced by capsular-like polysaccharide antigen of Actinobacillus actinomycetemcomitans strain Y4.
Role of prostaglandin in the formation of osteoclasts induced by capsular-like polysaccharide antigen of Actinobacillus actinomycetemcomitans strain Y4.
复制标题
前列腺素在伴放线放线杆菌Y4株荚膜样多糖抗原诱导破骨细胞形成中的作用。
DOI：
10.1111/j.1399-302x.1995.tb00121.x
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发表时间：
1995
影响因子：
--
通讯作者：
Toshihide Noguchi
中科院分区：
文献类型：
--
作者：
N. Ueda;Tatsuji Nishihara;Y. Ishihara;K. Amano;T. Kuroyanagi;Toshihide Noguchi
我们没有发现从放线菌中纯化的荚膜样多糖抗原在小鼠器官培养中诱导破骨细胞骨吸收或促进小鼠骨髓培养中破骨细胞形成的报道。相比之下，从放线菌Y4菌株纯化的荚膜样多糖抗原在小鼠器官培养中诱导骨吸收。为探讨放线菌诱导骨吸收的机制，用放线菌Y4荚膜样多糖抗原培养小鼠骨髓细胞。放线菌Y4荚膜样多糖抗原刺激小鼠骨髓培养破骨细胞样细胞形成。放线菌脂多糖不诱导破骨细胞样细胞的形成。吲哚美辛对放线菌Y4荚膜样多糖抗原介导的破骨细胞样细胞形成具有剂量依赖性。骨髓培养中形成的破骨细胞样细胞数量与前列腺素E2释放到培养基中的量有良好的相关性。用前列腺素E2培养小鼠骨髓细胞，可形成大量破骨细胞样细胞。上述结果提示前列腺素E2参与了A.放线菌Y4荚膜样多糖抗原介导的破骨细胞样细胞形成的机制。A.放线菌（actinomycetemcomitans）菌株Y4荚膜样多糖抗原可能通过促进破骨细胞形成在牙周病炎症性骨吸收中发挥重要作用。
We found no reports that capsular-like polysaccharide antigen purified from Actinobacillus actinomycetemcomitans either induces osteoclastic bone resorption in mouse organ cultures or promotes osteoclast formation in mouse marrow cultures. In contrast, capsular-like polysaccharide antigen purified from A. actinomycetemcomitans strain Y4 induced bone resorption in mouse organ culture. To examine the mechanism of bone resorption induced by A. actinomycetemcomitans, mouse bone marrow cells were cultured with A. actinomycetemcomitans strain Y4 capsular-like polysaccharide antigen. A. actinomycetemcomitans strain Y4 capsular-like polysaccharide antigen stimulated osteoclast-like cell formation in mouse bone marrow cultures. However, the polysaccharide of A. actinomycetemcomitans lipopolysaccharide did not induce the formation of osteoclast-like cells. Indomethacin inhibited osteoclast-like cell formation mediated by A. actinomycetemcomitans strain Y4 capsular-like polysaccharide antigen in a dose-dependent manner. There was a good correlation between the number of osteoclast-like cells formed in the marrow culture and the amount of prostaglandin E2 released into the culture media. When mouse bone marrow cells were cultured with prostaglandin E2 during the culture periods, many osteoclast-like cells were formed. These results indicate that prostaglandin E2 is involved in the mechanism of the formation of osteoclast-like cells mediated by A. actinomycetemcomitans strain Y4 capsular-like polysaccharide antigen. A. actinomycetemcomitans strain Y4 capsular-like polysaccharide antigen may play an important role in inflammatory bone resorption by promoting osteoclast formation in periodontal disease.
伴放线放线杆菌菌株 Y4 脂多糖对小鼠腹腔巨噬细胞的体外影响。
影响因子：
2.8
作者：
Barker,KA;Holt,SC
通讯作者：
Holt,SC