Lysosome-associated membrane glycoprotein 3 is involved in influenza A virus replication in human lung epithelial (A549) cells
Lysosome-associated membrane glycoprotein 3 is involved in influenza A virus replication in human lung epithelial (A549) cells
复制标题
溶酶体相关膜糖蛋白 3 参与人肺上皮 (A549) 细胞中甲型流感病毒的复制
作者：
Zhuo Zhou;Qinghua Xue;Y. Wan;Yaowu Yang;Jianwei Wang;T. Hung
背景甲型流感病毒突变迅速，导致针对病毒编码靶点的抗病毒疗法和疫苗无效。对流感病毒所利用的宿主因子和分子途径的了解将为新的抗病毒策略提供进一步的靶点。然而，参与流感病毒infection的关键宿主因素尚未完全defined.ResultsWe表明，LAMP 3，溶酶体相关膜糖蛋白（LAMP）家族的成员，显着诱导人肺上皮细胞（A549）流感病毒感染后。通过RNA干扰敲低LAMP3表达减弱了病毒核蛋白（NP）的产生以及病毒滴度。共聚焦显微镜结果表明，在病毒感染的早期阶段，病毒NP共定位在LAMP3阳性囊泡内。结论LAMP3基因是流感病毒的诱导基因，在流感病毒进入细胞后的过程中起重要作用。我们的观察结果可能会提供深入了解流感病毒复制的机制和潜在的新的抗流感治疗药物的目标。
BackgroundInfluenza A virus mutates rapidly, rendering antiviral therapies and vaccines directed against virus-encoded targets ineffective. Knowledge of the host factors and molecular pathways exploited by influenza virus will provide further targets for novel antiviral strategies. However, the critical host factors involved in influenza virus infection have not been fully defined.ResultsWe demonstrated that LAMP3, a member of lysosome-associated membrane glycoprotein (LAMP) family, was significantly induced in human lung epithelial (A549) cells upon influenza A virus infection. Knockdown of LAMP3 expression by RNA interference attenuated production of viral nucleoprotein (NP) as well as virus titers. Confocal microscopy results demonstrated that viral NP is colocalized within LAMP3 positive vesicles at early stages of virus infection. Furthermore, knockdown of LAMP3 expression led to a reduction in nuclear accumulation of viral NP and impeded virus replication.ConclusionsLAMP3 is an influenza A virus inducible gene, and plays an important role in viral post-entry steps. Our observations may provide insights into the mechanism of influenza virus replication and potential targets for novel anti-influenza therapeutics.
影响因子：
11.2
作者：
Kanao, H;Enomoto, T;Murata, Y
通讯作者：
Murata, Y
影响因子：
4.3
作者：
Kannan, K;Stewart, RM;Holcombe, RF
通讯作者：
Holcombe, RF
DOI：
10.1016/s0021-9258(18)37369-1
发表时间：
1988-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
S. Carlsson;J. Roth;F. Piller;Minoru Fukuda
通讯作者：
S. Carlsson;J. Roth;F. Piller;Minoru Fukuda