OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice
OsSPO11-1 is essential for both homologous chromosome pairing and crossover formation in rice
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DOI：
10.1007/s00412-010-0284-7
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发表时间：
2010-07
期刊：
影响因子：
1.6
通讯作者：
Hengxiu Yu;Mo Wang;Ding Tang;Kejian Wang;Fu-An Chen;Z. Gong;M. Gu;Zhukuan Cheng
中科院分区：
文献类型：
--
作者：
Hengxiu Yu;Mo Wang;Ding Tang;Kejian Wang;Fu-An Chen;Z. Gong;M. Gu;Zhukuan Cheng
Spo 11是古细菌拓扑异构酶亚基的同源物，其催化DNA双链断裂并启动同源染色体重组。本研究利用RNAi技术沉默水稻SPO 11 - 1基因。OsSPO 11 -1功能缺失的水稻植株在营养生长发育过程中没有明显的生长缺陷，但同源染色体配对和重组受到明显阻碍。在OsSPO 11 -1基因缺失的植株中，端粒在合子期可以组装成花束，与野生型一样。尽管两种轴相关蛋白REC 8和PAIR 2被加载到染色体上，但PAIR 2从染色体上的耗尽比野生型晚得多。联会复合体（SC）的中心元件ZEP 1不能正常加载到染色体上，这意味着SC的形成受到严重干扰。交换蛋白MER 3不能有效地组装到染色体上，并且缺乏二价体表明在缺乏OsSPO 11 -1的情况下交换也受到影响。因此，OsSPO 11 -1在水稻减数分裂过程中对同源染色体配对和交换形成都是必需的。
Spo11 is a homolog of a subunit of archaebacterial topoisomerase, which catalyzes DNA double-strand breaks and initiates homologous chromosome recombination. In the present study, we silenced theSPO11-1gene in rice (Oryza sativa) using RNAi. Rice plants with loss-of-function of OsSPO11-1 have no apparent growth defects during vegetative development, but homologous chromosome pairing and recombination are significantly obstructed. Telomeres can be assembled as bouquet during the zygotene stage of the OsSPO11-1-deficient plants, just as that in wild type. Although the two axial-associated proteins, REC8 and PAIR2, are loaded onto the chromosomes, the depletion of PAIR2 from the chromosomes is much later than in wild type. The central element of the synaptonemal complex (SC), ZEP1, does not load onto the chromosomes normally, implying that SC formation is disturbed severely. The crossover protein, MER3, isn't efficiently assembled onto chromosomes and the lack of bivalent suggests that crossovers are also affected in the absence of OsSPO11-1. Thus, OsSPO11-1 is essential for both homologous chromosomes pairing and crossover formation during meiosis in rice.