Development and characterization of 38 microsatellite markers for Camellia flavida based on transcriptome sequencing
Development and characterization of 38 microsatellite markers for Camellia flavida based on transcriptome sequencing
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DOI：
10.1007/s12686-014-0270-0
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发表时间：
2014-12-01
影响因子：
1.1
通讯作者：
Tang, Shao-Qing
中科院分区：
文献类型：
--
作者：
Liufu, Yong-Qing;Peng, Guo-Qing;Tang, Shao-Qing
黄花红山茶是一种珍贵的濒危观赏植物。在此，我们分离并鉴定了38个多态性微卫星位点。flavida使用转录组测序。对一个群体进行了检测。flavida和一个近缘的C. nitidissima。梭在florea中，所有位点均表现出多态性，每个位点的等位基因数在2 ~ 8个之间。观察到的和预期的杂合度范围分别为0.250至0.900和0.250至0.789。这些分子标记将为进一步研究中国对虾的遗传多样性和群体遗传结构提供依据。弗拉维达。这些基因座也可用于其它相关的山茶属物种。
Camellia flavida is an endangered and valuable ornamental plant species with yellow flower. Here we isolated and characterized 38 polymorphic microsatellite loci for C. flavida using transcriptome sequencing. The markers were tested on one population of C. flavida and one population of closely related C. nitidissima. For C. fladviia, all of these loci showed polymorphism, and the number of alleles per locus ranged from 2 to 8. The observed and expected heterozygosities ranged from 0.250 to 0.900 and from 0.250 to 0.789, respectively. These markers will be useful in the research on the genetic diversity and population genetic structure of C. flavida. These loci can also be used for other related Camellia species.