ASSEMBLY OF 2 TYPES OF TUBULES WITH PUTATIVE CYTOLYTIC FUNCTION BY CLONED NATURAL-KILLER CELLS
ASSEMBLY OF 2 TYPES OF TUBULES WITH PUTATIVE CYTOLYTIC FUNCTION BY CLONED NATURAL-KILLER CELLS
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DOI：
10.1038/302442a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
DENNERT, G
中科院分区：
文献类型：
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作者：
PODACK, ER;DENNERT, G
最近，Dourmashkinet等人和Henkartet等人报道了人外周血白细胞在细胞介导的细胞溶解过程中形成不同大小的超微结构膜损伤。以克隆的小鼠自然杀伤细胞(NK)3为效应物，以YAc-1细胞和兔红细胞为靶点，报道了两种类型的膜损伤，内径分别为16±2 nm和∼5 nm。这些损害是由于在细胞溶解反应中可能由亚基组装而成的管状复合体的膜插入引起的。免疫电子显微镜在靶膜上检测到这些小管，在超薄切片上可以看到它们形成跨膜通道。用十二烷基硫酸钠膜萃取和含有脱氧胆酸盐的缓冲液进行凝胶过滤，可以得到部分纯化的两条小管。根据小管组装和细胞溶解的相关性以及它们在靶膜上的检测，我们认为这两种类型的小管可能与细胞溶解有关。
The formation of ultrastructural membrane lesions of varying size during cell mediated cytolysis effected by human peripheral blood leukocytes was recently reported by Dourmashkinet al.1and Henkartet al.2. Using cloned mouse natural killer (NK) cells3as effectors and YAC-1 cells or rabbit erythrocytes as targets, we now report two types of membrane lesions with inner diameters of 16±2 nm and ∼5 nm, respectively. These lesions arise by membrane insertion of tubular complexes that may be assembled from subunits during the cytolytic reaction. The tubules are detected on target membranes by immune electron microscopy and appear to form transmembrane channels as seen in ultrathin sections. Both tubules are partially purified by membrane extraction with SDS and gel filtration in deoxycholate containing buffer. Based on the correlation of tubule assembly and cytolysis and on their detection on target membranes, we suggest that both types of tubules may be related to cytolysis.