IRMA (whole PTH) is a more useful assay for the effect of PTH on bone than the Allegro intact PTH assay in CAPD patients with low bone turnover marker.
IRMA (whole PTH) is a more useful assay for the effect of PTH on bone than the Allegro intact PTH assay in CAPD patients with low bone turnover marker.
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对于具有低骨转换标志物的 CAPD 患者，IRMA（全 PTH）是一种比 Allegro 完整 PTH 测定更有用的 PTH 对骨骼影响的测定方法。
DOI：
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发表时间：
2003
影响因子：
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通讯作者：
T. Hosoya
中科院分区：
文献类型：
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作者：
Y. Tanno;K. Yokoyama;M. Nakayama;Akihiko Katoh;Hiroyasu Yamamoto;Y. Iwasaki;T. Cantor;M. Fukagawa;T. Shigematsu;T. Hosoya
普通完整甲状旁腺激素(I-PTH)法不仅能检测PTH(1-84)，还能检测PTH(7-84)片段。最近有报道，PTH(7-84)片段是PTH(1-84)生物学作用的拮抗剂。另据报道，PTH(7-84)片段的积聚在血液透析(HD)患者的骨骼抵抗中发挥了作用。然而，PTH(7-84)片段积聚在持续不卧床腹膜透析(CAPD)患者中的作用仍不清楚，其清除率与HD患者不同。因此，我们用一种新的全甲状旁腺激素(w-PTH)测定方法对20例CAPD患者(男15例，女5例；平均年龄51.0±13.0岁)进行了甲状旁腺激素(1-84)活性测定。腹膜透析患者的w-PTH平均值为88.5+/-14.2pg/ml，是i-PTH(152.6+/-23.6pg/ml)的42.1%。用下式计算w-PTH的近似值(w-PTH=0.58xiPTH-0.4，R(2)=0.94)。PTH(7-84)片段按式I-PTH-w-PTH计算。甲状旁腺素(7-84)片段/w-甲状旁腺素比值作为骨骼阻力指标，与血清碱性磷酸酶活性作为成骨细胞标志物呈负相关(P=0.02)。根据这些结果，我们得出结论，用常规方法计算的甲状旁腺激素水平可能会导致CAPD患者与HD患者相似的甲状旁腺功能和骨转换的高估。W-甲状旁腺激素测定可能有助于更准确地评估终末期肾病患者的甲状旁腺素活性。
The common intact parathyroid hormone (i-PTH) assay detects not only PTH (1-84) but also the PTH (7-84) fragment. Recently, it was reported that the PTH (7-84) fragment is an antagonist to the biological action of PTH (1-84). It was also reported that the accumulation of the PTH (7-84) fragment plays a role in skeletal resistance in haemodialysis (HD) patients. However, the role of accumulation of the PTH (7-84) fragment in continuous ambulatory peritoneal dialysis (CAPD) patients, with a different clearance rate from that of HD patients, is still unclear. Therefore, we have measured only the active form of PTH (1-84) using a new method of whole PTH (w-PTH) assay in 20 CAPD patients (15 male and five female; mean age 51.0+/-13.0 years). The mean w-PTH value was 88.5 +/-14.2 pg/ml in CAPD patients, which was 42.1% of i-PTH (152.6+/-23.6 pg/ml). The approximate value of w-PTH was calculated using the following formula (w-PTH=0.58 x iPTH-0.4, R(2)=0.94). PTH (7-84) fragment was calculated by the formula i-PTH-w-PTH. The PTH (7-84) fragment/w-PTH ratio as an index of skeletal resistance, and serum alkaline phosphatase activity as an osteoblastic marker were negatively correlated (P=0.02). From these results, we concluded that the i-PTH level as calculated using the common assay method might lead to an overestimation of parathyroid function and bone turnover in CAPD patients similarly to HD patients. The w-PTH assay may be useful for more precise evaluation of PTH activity in end-stage renal disease patients.
影响因子：
19.6
作者：
Slatopolsky, E;Finch, J;Dusso, A
通讯作者：
Dusso, A
影响因子：
19.6
作者：
Monier-Faugere, MC;Geng, ZP;Malluche, HH
通讯作者：
Malluche, HH