Optimized High-Performance Liquid Chromatography Method for Determining Nine Cytokinins, Indole-3-acetic Acid and Abscisic Acid
Optimized High-Performance Liquid Chromatography Method for Determining Nine Cytokinins, Indole-3-acetic Acid and Abscisic Acid
复制标题
优化高效液相色谱法测定九种细胞分裂素、3-吲哚乙酸和脱落酸
DOI：
10.3390/su13136998
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发表时间：
2021-07-01
期刊：
影响因子：
3.9
通讯作者：
Tang, Hui
中科院分区：
文献类型：
--
作者：
Qi, Beibei;Wu, Chao;Tang, Hui
采用液-液萃取和固相萃取、高效液相色谱法和紫外检测器相结合的方法测定植物激素的含量。优化了影响HPLC-UV方法性能的参数，包括梯度洗脱的移动的相组成、柱温、流速和检测波长。该方法可同时测定9种细胞分裂素、吲哚乙酸和脱落酸等11种植物激素。该方法的检测限为0.22 - 1.1 µg L−1，线性回归系数>0.998。目标植物激素的加标回收率为62.1~ 109.4%，相对标准偏差<10%。该方法适用于测定水稻幼穗、根和木质部汁液中的植物激素，特别是细胞分裂素。
Liquid-liquid extraction and solid phase extraction followed by high-performance liquid chromatography (HPLC) connected with ultraviolet (UV) detection were used for the determination of phytohormones. The parameters influencing the performance of the HPLC-UV method, including composition of the mobile phase for gradient elution, column temperature, flow rate, and detection wavelength, were optimized. This method can simultaneously determine 11 phytohormones, including nine cytokinins, indole-3-acetic acid, and abscisic acid. The limit of detection of this method is 0.22 to 1.1 µg L−1, and the coefficient factors of linear regression are >0.998. The recoveries of the target phytohormones ranged between 62.1~109.4%, and the relative standard deviations were <10%. This method is suitable for determining phytohormones, especially cytokinins, in young panicles, roots, and xylem sap of rice plants.