Field effect of human colon carcinoma on normal mucosa: Relevance of carcinoembryonic antigen expression
Field effect of human colon carcinoma on normal mucosa: Relevance of carcinoembryonic antigen expression
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DOI：
10.1159/000217967
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发表时间：
1996-01-01
期刊：
影响因子：
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通讯作者：
Rabczynski, J
中科院分区：
文献类型：
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作者：
Jothy, S;Slesak, B;Rabczynski, J
人类结肠癌通常发生在已经经历了多个基因改变步骤的粘膜上。这些多个步骤产生了场效应，其特征在于存在形态正常但生物学改变的上皮细胞。本研究的目的是评估癌胚抗原（CEA）的表达是否可以作为场效应的表型标记，并绘制其与结直肠腺癌存在相关的拓扑图。通过免疫组织化学方法，在 14 例自体结直肠腺癌病例的 4 个递增距离处的形态正常粘膜上检测 CEA 的表达。将正常粘膜与肿瘤中的CEA表达进行比较，结果显示，在自体肿瘤边缘附近的粘膜中，CEA的表达水平与癌中显示的水平相同；距癌边缘1cm或更远的正常粘膜中CEA表达减少。距肿瘤 5 厘米和 10 厘米处取样的粘膜表达的 CEA 水平与无结直肠肿瘤的对照受试者粘膜中的水平相同。总之，本研究证明了肿瘤周围区域 CEA 表达的梯度，支持场效应的概念，并绘制了其范围。这些数据与人类结直肠癌的生物学相关，更实际地与手术切除的最佳位置相关。
Human colon cancer usually develops on a mucosa which has already undergone multiple steps of genetic change. These multiple steps create a Field effect characterized by the presence of morphologically normal, but biologically altered epithelial cells. This aims of this study were to evaluate whether the expression of carcinoembryonic antigen (CEA) can act as a phenotypic marker of the field effect, and to map its topography in relation to the presence of colorectal adenocarcinoma. The expression of CEA was tested by immunohistochemistry on morphologically normal mucosa at 4 increasing distances from 14 autologous cases of colorectal adenocarcinoma. CEA expression in the normal mucosa was compared to the tumor, The results show that in the mucosa adjacent to the edge of the autologous tumor, CEA is expressed to the same level as displayed in the carcinoma; there is a decrease in CEA expression in normal mucosa located at 1 cm or more from the edge of carcinoma. Mucosa sampled at 5 and 10 cm from the tumor expresses CEA at the same low level as in mucosa of control subjects with no colorectal neoplasm. In conclusion, this study demonstrates a gradient of CEA expression in the peritumoral area, supporting the concept of field effect, and maps its extent. These data are relevant to the biology of human colorectal cancer, and more practically, to the optimal location of surgical resection.