Simple and reliable method to precipitate proteins from bacterial culture supernatant
Simple and reliable method to precipitate proteins from bacterial culture supernatant
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DOI：
10.1128/aem.70.1.610-612.2004
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发表时间：
2004-01-01
影响因子：
4.4
通讯作者：
Lattemann, CT
中科院分区：
文献类型：
--
作者：
Caldwell, RB;Lattemann, CT
基于邻苯三酚红-钼酸盐-甲醇 (PRMM) 方案，开发了一种简单可靠的从细菌培养上清液中沉淀蛋白质的方法，并将其应用于分析 III 型细菌分泌系统分泌的蛋白质。基于 PRMM 的沉淀已被证明比传统方案更有效、更稳健。
A simple and reliable method for precipitating protein from bacterial culture supernatants based on a pyrogallol red-molybdate-methanol (PRMM) protocol has been developed and applied for the analysis of proteins secreted by a bacterial type III secretion system. PRMM-based precipitation has been shown to be more efficient and robust than are conventional protocols.