Effects of senescent lens epithelial cells on the severity of age-related cortical cataract in humans: A case-control study
Effects of senescent lens epithelial cells on the severity of age-related cortical cataract in humans: A case-control study
复制标题
衰老晶状体上皮细胞对人类年龄相关性皮质白内障严重程度的影响：病例对照研究
DOI：
10.1097/md.0000000000003869
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发表时间：
2016-06-01
期刊：
影响因子：
1.6
通讯作者：
Yao, Ke
中科院分区：
文献类型：
--
作者：
Fu, Qiuli;Qin, Zhenwei;Yao, Ke
透镜祖细胞的老化在年龄相关性白内障（age-related cataract，ARC）的发生发展中起着重要作用，但其机制尚不清楚。本研究旨在探讨人类透镜祖细胞/上皮细胞老化与ARC 4种亚型之间的关系。从ARC患者手术中收集的透镜囊，根据患者年龄分为3组（50-60岁，60-80岁和>80岁）。首先在人透镜上皮细胞（HLEC）中原位检查透镜祖细胞相关标志物Sox 2、Abcg 2和Ki 67的表达。然后，定量从透镜囊分离的衰老和SA--gal（+）HLEC的百分比。最后，分析了衰老（和SA--gal（+））HLEC百分比与ARC严重程度之间的潜在关系。透镜囊中Ki 67（+）、Sox 2（+）和Abcg 2（+）HLEC在年轻人中明显比在50岁以上患者中更丰富，而在60岁以上患者中几乎不存在。具有老化形态的原发性HLEC的百分比随年龄增加而增加，与SA-gal（+）原发性HLEC的结果一致。我们的研究为人透镜囊中透镜干/祖细胞随年龄的动态变化提供了初步证据，并提示透镜祖/上皮细胞的老化在皮质性白内障的严重程度中起重要作用。
The aging of lens progenitor cell has been repeatedly proposed to play a key role in age-related cataracts (ARCs), but the mechanism is far from being understood. The present study aims to investigate the relationship between aging of lens progenitor/epithelial cells and the 4 subtypes of ARCs in humans.Lens capsules, which were collected from ARC patients during surgery, were divided into 3 groups according to the age of patients (50-60, 60-80, and >80 years). The expressions of lens progenitor cell-related markers Sox2, Abcg2, and Ki67 were first examined in human lens epithelial cells (HLECs) in situ. Then, the percentage of senescent and SA--gal(+) HLECs isolated from lens capsules were quantified. Finally, the potential relationships between the percentage of senescent (and SA--gal(+)) HLECs and the severity of ARCs were analyzed.Ki67(+), Sox2(+), and Abcg2(+) HLECs in lens capsules were clearly more abundant in young people than in patients older than 50 years, and they were almost absent in patients older than 60 years. The percentage of primary HLECs with aging morphology increased with age, consistent with the results of SA--gal(+) primary HLECs. Only cortical cataract classification was found to be strongly related to the percentage of SA--gal(+) and senescent HLECs.Our study gave the initial evidence on the dynamical change of lens stem/progenitor cells in human lens capsule with age and suggested that lens progenitor/epithelial cell aging is important in the severity of cortical cataracts.