Genome-wide changes in histone H3 lysine 27 trimethylation associated with bud dormancy release in peach
Genome-wide changes in histone H3 lysine 27 trimethylation associated with bud dormancy release in peach
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DOI：
10.1007/s11295-015-0869-7
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发表时间：
2015-06-01
影响因子：
2.4
通讯作者：
Rios, Gabino
中科院分区：
文献类型：
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作者：
de la Fuente, Lorena;Conesa, Ana;Rios, Gabino
芽休眠是多年生植物对温带气候温度季节性波动的一种进化适应，受内在和环境信号的影响。最近的研究指出了表观遗传机制在芽休眠调节中的相关作用。我们对桃（Prunus persica）休眠(D)和休眠释放（ND）芽中的组蛋白H3赖氨酸-27三甲基化（H3K27me3）进行了染色质免疫沉淀测序（ChIP-seq）分析，这是一个与稳定基因沉默相关的染色质标记。H3K27me3区域在染色体富含基因的同染色质区更为丰富，并与基因体相关。在ND样本中发现休眠调节因子dormancy- associated MADS-box (DAM) 1、DAM4、DAM5和DAM6在H3K27me3中显著富集，与其休眠特异性表达密切一致。DAM基因座在特定的短区域被修饰，允许不同的DAM基因不均匀调节。拟南芥中与分生组织活性和开花基因相关的其他调控因子H3K27三甲基化存在差异，表明桃生殖芽中分生组织的再激活和开花发育也可能受到表观遗传调控。发现A (GA)n基序和cta型转座子相关序列在H3K27me3区域中大量存在。
Bud dormancy is an evolutionary adaptation of perennial plants to the seasonal fluctuation of temperatures in temperate climates, affected by intrinsic and environmental signals. Recent investigations point to a relevant role of epigenetic mechanisms in the regulation of bud dormancy. We have performed a chromatin immunoprecipitation sequencing (ChIP-seq) analysis of histone H3 lysine-27 trimethylation (H3K27me3), a chromatin mark associated with stable gene silencing, in dormant (D) and dormancy-released (ND) buds of peach (Prunus persica). H3K27me3 regions were more abundant in gene-rich euchromatic zones of chromosomes and associated with gene bodies. The dormancy regulators DORMANCY-ASSOCIATED MADS-box (DAM) 1, DAM4, DAM5 and DAM6 were found significantly enriched in H3K27me3 in ND samples, in close agreement with their dormancy-specific expression. The DAM locus was modified at specific short regions, allowing the uneven regulation of distinct DAM genes. Additional regulatory factors related to meristem activity and flowering genes from Arabidopsis thaliana were differentially H3K27 trimethylated, which suggests that meristem reactivation and flower development could be also epigenetically regulated in reproductive buds of peach. A (GA)n motif and CACTA-type transposon-related sequences were found over-represented in H3K27me3 regions.