Time-dependent analysis of nociception and anxiety-like behavior in rats submitted to persistent inflammation of the temporomandibular joint
Time-dependent analysis of nociception and anxiety-like behavior in rats submitted to persistent inflammation of the temporomandibular joint
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DOI：
10.1016/j.physbeh.2013.11.009
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发表时间：
2014-02-10
影响因子：
2.9
通讯作者：
Andrade Leite-Panissi, Christie Ramos
中科院分区：
文献类型：
--
作者：
do Nascimento, Glance Crivelaro;Andrade Leite-Panissi, Christie Ramos
颞下颌关节紊乱病(TMD)在牙科临床中很常见，可能涉及咀嚼肌或颞下颌关节(TMJ)的问题。TMD的疼痛通常与TMJ的炎症有关，但其病因被认为是多因素的，包括生物、行为、环境、社会、情感和认知因素。本研究通过高架迷宫(EPM)和明暗盒(LDB)试验观察注射弗氏佐剂(CFA)后大鼠的焦虑样行为，并用von Frey试验评价不同时期大鼠的伤害性行为。此外，本研究通过血浆外渗(伊文思蓝试验)和关节内中性粒细胞浸润(髓过氧化物酶定量)来测量TMJ炎症。结果显示，受到TMJ炎症的大鼠进入EPM张开的手臂的次数减少，在光室中花费的时间和在LDB中的转换次数减少。此外，EPM中用作运动活动指示器的闭合手臂的条目数量在不同治疗之间没有改变。此外，在整个炎症过程中，关节组织机械敏感性的增加和血浆外渗的增加，以及TMJ滑液中髓过氧化物酶的增加。我们的结果表明，CFA诱导的大鼠颞颌关节炎症在不同的炎症阶段都会产生焦虑样行为，并诱导出伤害性行为。(C)2013 Elsevier Inc.保留所有权利。
Temporomandibular disorder (TMD) is prevalent in dental clinics and can involve problems with the masticatory muscles or the temporomandibular joints (TMJ). The pain of TMD is frequently associated with inflammation in the TMJs, but it's etiology is considered to be multifactorial and includes biologic, behavioral, environmental, social, emotional and cognitive factors. The purpose of this investigation was to evaluate the anxiety-like behavior in rats exposed to temporomandibular inflammation via injection of Freund's Adjuvant (CFA) with the elevated plus maze (EPM) and light/dark box (LDB) tests and to evaluate nociceptive behavior with the von Frey test at different periods. Moreover, this study measured TMJ inflammation using plasma extravasation (Evans blue test) and the intraarticular infiltration of polymorphonuclear neutrophils (myeloperoxidase quantification). The results showed that rats that were submitted to TMJ inflammation exhibited a decreased number of entries into the open arms of the EPM and a decrease in the time spent in the light compartment and in the number of transitions in the LDB. Additionally, the number of entries in closed arms in the EPM, used as indicator of locomotor activity, did not alter between treatments. Furthermore, increases in mechanical sensitivity and increases in plasma extravasation in the joint tissue occurred throughout the inflammation process, along with an increase in myeloperoxidase in the synovial fluid of TMJ. Our results suggest that the temporomandibular inflammation induced by CFA produced anxiety-like behaviors in rats and induced nociceptive behavior across different periods of inflammation. (C) 2013 Elsevier Inc. All rights reserved.