Graded-field microscopy with white light
Graded-field microscopy with white light
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DOI：
10.1364/oe.14.005191
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发表时间：
2006-06-12
期刊：
影响因子：
3.8
通讯作者：
Mertz, Jerome
中科院分区：
文献类型：
--
作者：
Yi, Ran;Chu, Kengyeh K.;Mertz, Jerome
我们提出了一种通用的成像技术，称为梯度场显微镜获得生物组织切片的相位梯度对比。该技术是基于引入部分光束块在照明和检测孔径的标准白光宽视场透照显微镜。根据相对孔径大小，一个块产生相位梯度对比度，而另一个块减少明场背景，从而允许明场和暗场对比度之间的整个操作范围。我们展示了大鼠脑切片中神经元的梯度场成像。(c)2006年美国光学学会。
We present a general imaging technique called graded-field microscopy for obtaining phase-gradient contrast in biological tissue slices. The technique is based on introducing partial beam blocks in the illumination and detection apertures of a standard white-light widefield transillumination microscope. Depending on the relative aperture sizes, one block produces phase-gradient contrast while the other reduces brightfield background, allowing a full operating range between brightfield and darkfield contrast. We demonstrate graded-field imaging of neurons in a rat brain slice. (c) 2006 Optical Society of America.