Requirement of the Yersinia pseudotuberculosis effectors YopH and YopE in colonization and persistence in intestinal and lymph tissues
Requirement of the Yersinia pseudotuberculosis effectors YopH and YopE in colonization and persistence in intestinal and lymph tissues
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DOI：
10.1128/iai.71.8.4595-4607.2003
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发表时间：
2003-08-01
影响因子：
3.1
通讯作者：
Mecsas, J
中科院分区：
文献类型：
--
作者：
Logsdon, LK;Mecsas, J
革兰氏阴性肠道病原体假结核耶尔森氏菌采用III型分泌系统和毒力所需的效应子Yop蛋白。III型分泌易位装置中的突变已显示导致小鼠盲肠定殖缺陷，表明肠道中一个或多个效应子Yop的作用。为了研究这种可能性，测试了同基因yop突变株在口胃接种后在小鼠肠道和相关淋巴组织中定殖和持续存在的能力。在单菌株感染中，yopHEMOJ突变株无法在肠道和淋巴组织中定植、复制或持续存在。yopH突变株特异性地不能定殖于肠系膜淋巴结，但yopE和yopO突变株在肠和淋巴组织中的持久性仅显示出微小的缺陷。虽然没有发现单一的Yop是在单菌株感染中在肠组织中定殖或持久性所必需的，但YopH和YopE两者的缺失一起几乎消除了所有组织的定殖，这表明这两种Yop具有一些冗余功能，或者Y.假结核病采用多种定植策略。在与野生型Y.在假结核病中，野生型细菌的存在严重阻碍了yopH、yopE和yopO突变体在许多组织中持续存在的能力，这表明野生型细菌填充定殖小生境或激发yop突变体无法承受的宿主应答。
The gram-negative enteric pathogen Yersinia pseudotuberculosis employs a type III secretion system and effector Yop proteins that are required for virulence. Mutations in the type III secretion-translocation apparatus have been shown to cause defects in colonization of the murine cecum, suggesting roles for one or more effector Yops in the intestinal tract. To investigate this possibility, isogenic yop mutant strains were tested for their ability to colonize and persist in intestinal and associated lymph tissues of the mouse following orogastric inoculation. In single-strain infections, a yopHEMOJ mutant strain was unable to colonize, replicate, or persist in intestinal and lymph tissues. A yopH mutant strain specifically fails to colonize the mesenteric lymph nodes, but yopE and yopO mutant strains showed only minor defects in persistence in intestinal and lymph tissues. While no single Yop was found to be essential for colonization or persistence in intestinal tissues in single-strain infections, the absence of both YopH and YopE together almost eliminated colonization of all tissues, indicating either that these two Yops have some redundant functions or that Y. pseudotuberculosis employs multiple strategies for colonization. In competition infections with wild-type Y. pseudotuberculosis, the presence of wild-type bacteria severely hindered the ability of the yopH, yopE, and yopO mutants to persist in many tissues, suggesting that the wild-type bacteria either fills colonization niches or elicits host responses that the yop mutants are unable to withstand.