Molecular engineering of a high quantum yield NIR-II molecular fluorophore with aggregation-induced emission (AIE) characteristics for in vivo imaging.
Molecular engineering of a high quantum yield NIR-II molecular fluorophore with aggregation-induced emission (AIE) characteristics for in vivo imaging.
复制标题
具有聚集诱导发射 (AIE) 特性的高量子产率 NIR-II 分子荧光团的分子工程，用于体内成像。
DOI：
10.1039/c9nr09999a
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发表时间：
2020
期刊：
影响因子：
6.7
通讯作者：
Wang Jing
中科院分区：
文献类型：
--
作者：
Xu Pengfei;Kang Fei;Yang Weidong;Zhang Mingru;Dang Ruili;Jiang Pei;Wang Jing
NIR-II生物成像（1000-1700 nm）已显示出深层组织穿透、高空间分辨率和低背景噪声的前景。在所有的近红外探针中，有机探针由于其优异的稳定性和生物相容性而引起了人们的极大关注，这是最有潜力的临床翻译。然而，大多数先前报道的有机NIR-II荧光剂在水溶液中经常遭受低量子产率。在此，我们开发了一种新的D-π-A-π-D型近红外II发色团XA 1的AIE特性的基础上，一种新的设计策略的近红外II AIE荧光团。由于其固有的聚集诱导的发射增强性质，配制的XA 1纳米颗粒显示出高达14.8%的高荧光量子产率，这高于大多数先前报道的有机NIR-II荧光团。基于XA 1纳米颗粒，实现了肢体和脑血管的无创成像，具有高信号背景比和深穿透性。此外，XA 1纳米颗粒可用作良好的造影剂，用于肿瘤血管的高分辨率成像和基于EPR机制的肿瘤的精确检测。总的来说，我们的工作证明了一种设计和制造具有AIE特性的NIR-II荧光团的新策略，并证明XA 1 NPs是非常有前途的NIR-II探针，用于生理和病理条件下的生物医学成像。
NIR-II biological imaging (1000–1700 nm) has shown promise for deep tissue penetration, high spatial resolution, and low background noise. Among all the NIR-II probes, organic probes particularly attract huge attention due to their excellent stability and biocompatibility, which have the most potential for clinical translation. However, most previously reported organic NIR-II fluorescent agents often suffer from low quantum yields in aqueous solution. Herein, we developed a novel D–π-A–π-D-type NIR II chromophore XA1 with AIE characteristics based on a new design strategy for NIR-II AIE fluorophores. Owing to their intrinsic aggregation-induced emission enhancement nature, the formulated XA1 NPs show a high fluorescence quantum yield up to 14.8%, which is higher than those of most previously reported organic NIR-II fluorophores. Based on the XA1 NPs, noninvasive imaging of limb and cerebral vessels is achieved with a high signal-to-background ratio and deep penetration. Furthermore, the XA1 NPs can be used as good contrast agents for high resolution imaging of blood vessels of tumors and precise detection of tumors based on the EPR mechanism. Collectively, our work demonstrated a novel strategy for designing and manufacturing NIR-II fluorophores with AIE characteristics and proved that XA1 NPs are highly promising NIR-II probes for biomedical imaging under physiological and pathological conditions.