Let-7b regulates the expression of the growth hormone receptor gene in deletion-type dwarf chickens.
Let-7b regulates the expression of the growth hormone receptor gene in deletion-type dwarf chickens.
复制标题
Let-7b调控缺失型矮鸡生长激素受体基因的表达
DOI：
10.1186/1471-2164-13-306
复制
发表时间：
2012-07-10
期刊：
影响因子：
4.4
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Lin S;Li H;Mu H;Luo W;Li Y;Jia X;Wang S;Jia X;Nie Q;Li Y;Zhang X
研究背景生长激素受体（growth hormone receptor，GHR）基因缺失突变导致矮小鸡骨骼肌生长和脂肪沉积受到抑制。我们利用基因芯片技术检测了14日龄胚胎、7周龄缺失型侏儒鸡和正常型鸡骨骼肌中GHR的microRNA（miRNA）和mRNA表达谱。我们的目的是阐明生长抑制和脂肪deposition.ResultsAt相同的发育阶段，不同的表达谱发生在骨骼肌的侏儒和正常鸡四个miRNA（miR-1623，miR-181 b，let-7 b，和miR-128）的GHR表达的miRNA调控。在不同的发育阶段，有一个显着差异的表达谱的更多的miRNA。与14日龄胚胎相比，7周龄侏儒鸡中有11种miRNA表达上调，18种下调。与14日龄胚胎相比，7周龄正常鸡中有7个miRNAs表达上调，9个表达下调。在骨骼肌中，与7周龄侏儒鸡相比，14日龄胚胎中有22个基因表达上调，33个基因表达下调。与7周龄正常鸡相比，14日龄鸡胚中有65个mRNA表达上调，108个mRNA表达下调。根据种子序列和靶序列的重叠，将34种差异表达的miRNAs分为18类。只有let-7 b与GHR的3′端非翻译区互补，能抑制GHR的表达。京都基因百科全书和基因组通路分析和定量聚合酶链反应表明，调控鸡骨骼肌生长和脂肪沉积的信号通路主要有3条。这些都受到let-7 b调节的GHR的影响。结论let-7 b是GHR调控的关键miRNA，SOCS 3通过let-7 b介导的GHR表达在骨骼肌生长和脂肪沉积中起重要作用。
BackgroundA deletion mutation in the growth hormone receptor (GHR) gene results in the inhibition of skeletal muscle growth and fat deposition in dwarf chickens. We used microarray techniques to determine microRNA (miRNA) and mRNA expression profiles ofGHRin the skeletal muscles of 14-day-old embryos as well as 7-week-old deletion-type dwarf and normal-type chickens. Our aim was to elucidate the miRNA regulation ofGHRexpression with respect to growth inhibition and fat deposition.ResultsAt the same developmental stages, different expression profiles in skeletal muscles of dwarf and normal chickens occurred for four miRNAs (miR-1623, miR-181b, let-7b, and miR-128). At different developmental stages, there was a significant difference in the expression profiles of a greater number of miRNAs. Eleven miRNAs were up-regulated and 18 down-regulated in the 7-week-old dwarf chickens when compared with profiles in 14-day-old embryos. In 7-week-old normal chickens, seven miRNAs were up-regulated and nine down-regulated compared with those in 14-day-old embryos. In skeletal muscles, 22 genes were up-regulated and 33 down-regulated in 14-day-old embryos compared with 7-week-old dwarf chickens. Sixty-five mRNAs were up-regulated and 108 down-regulated in 14-day-old embryos as compared with 7-week-old normal chickens. Thirty-four differentially expressed miRNAs were grouped into 18 categories based on overlapping seed and target sequences. Only let-7b was found to be complementary to its target in the 3′ untranslated region ofGHR, and was able to inhibit its expression. Kyoto Encyclopedia of Genes and Genomes pathway analysis and quantitative polymerase chain reactions indicated there were three main signaling pathways regulating skeletal muscle growth and fat deposition of chickens. These were influenced by let-7b-regulatedGHR. Suppression of the cytokine signaling 3 (SOCS3) gene was found to be involved in the signaling pathway of adipocytokines.ConclusionsThere is a critical miRNA, let-7b, involved in the regulation ofGHR.SOCS3plays a critical role in regulating skeletal muscle growth and fat depositionvialet-7b-mediatedGHRexpression.
登录
查看更多内容
影响因子：
5.4
作者：
Fassone, L.;Corneli, G.;Bona, G.
通讯作者：
Bona, G.
影响因子：
4.8
作者：
Coschigano, KT;Holland, AN;Kopchick, JJ
通讯作者：
Kopchick, JJ
影响因子：
4.8
作者：
Freeth, JS;Ayling, RM;Clayton, PE
通讯作者：
Clayton, PE
影响因子：
4
作者：
AGARWAL, SK;COGBURN, LA;BURNSIDE, J
通讯作者：
BURNSIDE, J
影响因子：
30.8
作者：
Clop, Alex;Marcq, Fabienne;Georges, Michel
通讯作者：
Georges, Michel