Presence and characterization of multiple mantle lysozymes in the Pacific oyster, Crassostrea gigas.
Presence and characterization of multiple mantle lysozymes in the Pacific oyster, Crassostrea gigas.
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DOI：
10.1016/j.fsi.2010.02.027
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发表时间：
2010-07
影响因子：
4.7
通讯作者：
N. Itoh;Yuki Okada;Keisuke G. Takahashi;M. Osada
中科院分区：
文献类型：
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作者：
N. Itoh;Yuki Okada;Keisuke G. Takahashi;M. Osada
太平洋牡蛎（Crassostrea gigas）的外套膜组织提取物在较宽的 pH 范围内表现出抗革兰氏阳性细菌和溶菌酶活性，表明存在多种防御性外套膜溶菌酶。简并逆转录PCR检测到两种地幔溶菌酶CGL-1和一种新型溶菌酶CGL-3的表达，证实了地幔中存在多种溶菌酶。由于CGL-3是消化溶菌酶CGL-2的同源蛋白，因此推测CGL-3已经专门进化出防御功能。使用重组 CGL-1 和 CGL-3 进行的功能测定表明，CGL-1 和 CGL-3 在地幔组织中发挥着主要的防御作用，并且它们负责不同 pH、离子强度和温度条件下的溶菌酶活性。基于这些观察，我们得出结论，太平洋牡蛎中的多种套膜溶菌酶比单一溶菌酶在更广泛的条件下更适合宿主防御。
Mantle tissue extracts from the Pacific oyster, Crassostrea gigas, exhibited anti-Gram-positive bacterial and lysozyme activities over a wide pH range, suggesting that multiple defensive mantle lysozymes were present. Degenerated reverse-transcription PCR detected the expression of two mantle lysozymes, CGL-1 and a novel lysozyme CGL-3, confirming the presence of multiple lysozymes in the mantle. Since CGL-3 is a cognate protein of the digestive lysozyme CGL-2, it is assumed that CGL-3 has evolved specifically a defensive function. Functional assays using recombinant CGL-1 and CGL-3 suggested that CGL-1 and CGL-3 play a major defensive role in the mantle tissue, and that they are responsible for lysozyme activity under different pH, ionic strength and temperature conditions. Based on these observations, we conclude that multiple mantle lysozymes in the Pacific oyster are better for host-defense under broader conditions than a single lysozyme.