Hepatoprotective and antioxidant effects of dietary Angelica sinensis extract against carbon tetrachloride-induced hepatic injury in Jian Carp (Cyprinus carpio var. Jian)
Hepatoprotective and antioxidant effects of dietary Angelica sinensis extract against carbon tetrachloride-induced hepatic injury in Jian Carp (Cyprinus carpio var. Jian)
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DOI：
10.1111/are.12643
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发表时间：
2016-06-01
影响因子：
2
通讯作者：
Yin, Guojun
中科院分区：
文献类型：
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作者：
Cao, Liping;Du, Jinliang;Yin, Guojun
本研究旨在评价当归提取物(ASE)对四氯化碳(CCl4)诱导的建鲤肝毒性的保护作用。在饲料中分别添加0%、0.1%、0.5%和1.0%的ASE，连续60d后，按0.05mL/10g体重的比例向鱼体内注射30%CCl4橄榄油。注射后72h，取血和肝组织进行生化分析、彗星试验、组织病理学检查和细胞色素P3A基因表达。结果表明，饲料中添加0.1%、0.5%和1.0%的ASE可显著抑制CCl4诱导的谷丙转氨酶(GPT)和谷草转氨酶(GOT)的升高。0.5%和1.0%ASE对CCl_4所致小鼠血清乳酸脱氢酶(LDH)升高、总蛋白(TP)和白蛋白(Alb)降低也有抑制作用。在肝组织中，1.0%ASE可显著抑制CCl4诱导的肝组织丙二醛(MDA)生成和总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)及细胞色素P3A(CyP3A)基因表达的降低。彗星实验表明，0.5%和1.0%ASE处理组的尾矩、橄榄尾矩、尾长和尾部DNA%均呈正相关。0.5%和1.0%ASE可明显减轻CCl_4所致的组织学改变。总体而言，研究结果证明ASE具有剂量依赖性的保肝作用，并支持将ASE(1.0%)用作鱼的保肝和抗氧化剂。
The present study aimed to evaluate the hepatoprotective effects of Angelica sinensis extract (ASE) against carbon tetrachloride (CCl4)-induced hepatotoxicity in Jian carp (Cyprinus carpio var.Jian). Fish were fed diets containing four doses of ASE (0%, 0.1%, 0.5% and 1.0%) for 60 days, and then given an intraperitoneal injection of 30% CCl4 in olive oil at a volume of 0.05 mL/10 g body weight. At 72 h post injection, blood and liver samples were collected for biochemical analysis, comet assay, histopathological examination and CYP3A mRNA expression. Results showed that the increases of glutamate pyruvate transaminase (GPT) and glutamate oxalate transaminase (GOT) induced by CCl4 were significantly inhibited by pre-treating the fish with 0.1%, 0.5% and 1.0% ASE in the diets. The elevation of lactate dehydrogenase (LDH) and the reductions of the total protein (TP) and albumin (Alb) in the serum induced by CCl4 were also inhibited by pre-treatments with 0.5 and 1.0% ASE. In the liver tissue, pre-treatment with 1.0% ASE significantly inhibited the malondialdehyde (MDA) formation and the reductions of the total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione (GSH) and CYP3A mRNA expression induced by CCl4. Comet assay showed that tail moment, olive tail moment, tail length and tail DNA% were positively changed in fish pretreated with 0.5 and 1.0% ASE. CCl4-induced histological changes were obviously reduced by 0.5% and 1.0% ASE. Overall results prove the hepatoprotective effect of ASE in a dose-dependent manner and support the use of ASE (1.0%) as a hepatoprotective and antioxidant agent in fish.