Determination of kanamycin A, amikacin and tobramycin residues in milk by capillary zone electrophoresis with post-column derivatization and laser-induced fluorescence detection
Determination of kanamycin A, amikacin and tobramycin residues in milk by capillary zone electrophoresis with post-column derivatization and laser-induced fluorescence detection
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柱后衍生毛细管区带电泳和激光诱导荧光检测法测定牛奶中卡那霉素 A、阿米卡星和妥布霉素残留量
DOI：
10.1016/j.jchromb.2008.12.011
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发表时间：
2009-01-15
影响因子：
3
通讯作者：
Gan, Wu-Er
中科院分区：
文献类型：
--
作者：
Yu, Chang-Zhu;He, You-Zhao;Gan, Wu-Er
建立了牛奶中氨基糖苷类抗生素卡那霉素A、阿米卡星和妥布霉素的毛细管区带电泳柱后衍生激光诱导荧光检测方法。采用自制的同轴缝隙反应器，在35 mmol/L的pH10.0的30%（v/v）甲醇四硼酸钠缓冲液中，以1.0mmol/L的2，3-萘二甲醛和8.0mmol/L的2-巯基乙醇为衍生化试剂，对AGs进行柱后衍生。以含0.5 mmol/L十六烷基三甲基溴化铵的50 mmol/L乙酸钠（pH 5.0）为分离缓冲液。线性范围为2.1 × 10 ~（-5）~ 5.0 × 10 ~（-2）g/L，检出限为7 × 10 ~（-6）~ 2 × 10 ~（-5）g/L。牛奶样品中AGs的回收率为81.6%~ 93.1%（n = 3）。(c)2008 Elsevier B. V.保留所有权利。
An analytical method for kanamycin A, amikacin and tobramycin of aminoglycoside (AG) antibiotics in milk samples was proposed using capillary zone electrophoresis (CZE) with post-column derivatization and laser-induced fluorescence detection. A simple and convenient homemade coaxial-gap reactor was adopted in the post-column derivatization of the AGs with 1.0 mmol/L naphthalene-2,3-dicarboxaldehyde and 8.0 mmol/L 2-mercaptoethanol in 35 mmol/L sodium tetraborate buffer (pH 10.0) of 30% (v/v) methanol. 50 mmol/L sodium acetate (pH 5.0) containing 0.5 mmol/L cetyltrimethyl ammonium bromide was used as the separation buffer. The linear calibration concentrations and detection limits were from 2.1 X 10(-5) to 5.0 x 10(-2) g/L and in the range of 7 x 10(-6) to 2 x 10(-5) g/L, respectively. The recoveries of the AGs in milk samples were from 81.6 to 93.1% (n = 3). (c) 2008 Elsevier B.V. All rights reserved.