Detection of tyramine and tyrosinase activity using red region emission NaGdF4:Yb,Er@NaYF4 upconversion nanoparticles
Detection of tyramine and tyrosinase activity using red region emission NaGdF4:Yb,Er@NaYF4 upconversion nanoparticles
复制标题
使用红色区域发射 NaGdF4:Yb,Er@NaYF4 上转换纳米颗粒检测酪胺和酪氨酸酶活性
DOI：
10.1016/j.talanta.2019.01.079
复制
发表时间：
2019
期刊：
影响因子：
6.1
通讯作者：
Chen Hongqi
中科院分区：
文献类型：
--
作者：
Wang Huihui;Lu Yunyun;Wang Lun;Chen Hongqi
利用光诱导电子转移（PET）机制，在上转换纳米粒子（UCNPs）和类黑色素聚合物之间建立了一种选择性、简单、环境友好的荧光传感平台。类黑色素聚合物对UCNPs的有效荧光猝灭可以通过生物催化氧化酪胺生成类黑色素聚合物产物来检测酪胺和酪氨酸酶（TYR）活性。在最佳条件下，酪胺的检测线性范围为0.167-33.3 μ M，酪胺的检测限为0.026 μ M。TYR的线性范围为0.0033 ~ 1 U·mL-1，检出限为0.003 U·mL-1。该方法应用于真实的样品分析，结果令人满意。
A selective, simple and environment-friendly type fluorescence sensing platform between upconversion nanoparticles (UCNPs) and melanin-like polymer utilizing the photoinduced electron-transfer (PET) mechanism was developed. The effective fluorescence quenching of UCNPs by melanin-like polymers could detect tyramine and tyrosinase (TYR) activity through the bio-catalysed oxidation of tyramine to the melanin-like polymer products. At optimal conditions, the range of linearity in detection was 0.167–33.3 μΜ for tyramine, and the limit of detection of tyramine was 0.026 μΜ. The linear range of TYR was 0.0033–1 U·mL−1, and the limit of detection of TYR was 0.003 U·mL−1. Furthermore, the strategy could be applied to real sample analysis with satisfactory results.