Let sleeping rats lie: Does the timing of husbandry procedures affect laboratory rat behaviour, physiology and welfare?
Let sleeping rats lie: Does the timing of husbandry procedures affect laboratory rat behaviour, physiology and welfare?
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DOI：
10.1016/j.applanim.2007.06.019
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发表时间：
2008-06-01
影响因子：
2.3
通讯作者：
Mendl, Michael
中科院分区：
文献类型：
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作者：
Abou-Ismail, Usama A.;Burman, Oliver H. P.;Mendl, Michael
研究表明，慢性压力会降低睡眠质量和数量。然而，很少有人调查动物正常睡眠期间进行的饲养程序是否会影响随后的睡眠行为和福利。我们将48只大鼠饲养在含有4只大鼠的富集笼中，在亮相处理（LPT）（n = 6笼）或暗相处理（DPT）（n = 6笼）中。LPT中的大鼠在光照阶段暴露于饲养程序（例如称重、清洁），我们预计它们将处于不活动/睡眠状态，每周三次。DPT大鼠经历了相同的程序，但在黑暗阶段，当我们希望他们是活跃/清醒的。5周后，在12 μ l光照和黑暗阶段对所有笼的行为进行采样。在5周饲养期内收集一般健康指标（例如体重），并在死后记录器官重量（例如胸腺）。主成分分析产生了四个因素，其中两个显示治疗差异。一方面，在血泪症评分上加载阳性，而在睡眠、自我梳理和丰富定向行为上加载阴性，LPT大鼠的评分高于DPT大鼠。另一个加载积极的相对胸腺重量和活动和消极的侵略和社会交往。DPT大鼠在这方面的得分高于LPT大鼠。因此，相对于DPT大鼠，LPT大鼠显示出福利降低的指标（例如睡眠减少、血泪增多、胸腺变轻、攻击性更高）。因此，在黑暗中而不是在光照阶段应用饲养程序可能会改善实验室大鼠的福利。(C)2007 Elsevier B. V.保留所有权利。
Research has indicated that chronic stress can reduce sleep quality and quantity. Yet there has been little investigation into whether husbandry procedures carried out during an animal's normal sleeping period affect subsequent sleep behaviour and welfare. We housed 48 rats in enriched cages containing four rats, in either a light phase treatment (LPT) (n = 6 cages) or a dark phase treatment (DPT) (n = 6 cages). Rats in the LPT were exposed to husbandry procedures (e.g. weighing, cleaning) during the light phase when we would expect them to be inactive/sleeping, three times every week. DPT rats experienced the same procedures, but during the dark phase when we would expect them to be active/awake. After five weeks, behaviour for all cages was sampled over 12 It of both the light and dark phase. General health measures (e.g. body weight) were collected over the five weeks housing period, and organ weights (e.g. thymus) were recorded post-mortem. Principal component analyses yielded four factors, two of which revealed treatment differences. For one, loading positively on chromodacryorrhoea score and negatively on sleep, self-grooming and enrichment directed behaviour, LPT rats scored higher than DPT rats. The other loaded positively for relative thymus weight and activity and negatively for aggression and social interaction. DPT rats scored higher on this than LPT rats. LPT rats thus displayed indicators of reduced welfare (e.g. less sleep, elevated chromodacryorrhoea, lighter thymus glands, higher aggression) relative to DPT rats. Therefore, husbandry procedures applied in the dark rather than the light phase might improve the welfare of laboratory rats. (C) 2007 Elsevier B.V. All rights reserved.