Evaluation of the stability of an SR398/GroES chaperonin complex
Evaluation of the stability of an SR398/GroES chaperonin complex
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SR398/GroES 伴侣蛋白复合物的稳定性评估
DOI：
10.1093/jb/mvu009
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
K. Matsuzaki & M. Hoshino.
中科院分区：
文献类型：
--
作者：
S. Ishino;Y. Kawata;T. Ikegami;K. Matsuzaki & M. Hoshino.
检查了SR 398/GroES伴侣蛋白复合物的稳定性。正如预期的那样，基于先前研究的发现，SR 398/GroES复合物在存在过量游离腺苷5′-[γ-硫代]三磷酸（ATPγS）或腺苷5′-（β，γ-亚氨基）三磷酸（AMPPNP）的情况下极其稳定。然而，该复合物在不存在核苷酸的情况下是不稳定的。这些结果表明ATPγS和AMPPNP以非合作的方式反复与复合物结合和解离。这种核苷酸交换没有诱导GroES和底物从SR 398解离，这表明在GroEL/GroES反应循环中核苷酸从膜环上协同解离以释放GroES和底物蛋白的重要性。
The stability of an SR398/GroES chaperonin complex was examined. As was expected, based on the finding of previous studies, the SR398/GroES complex was extremely stable in the presence of an excess amount of free adenosine 5′-[γ-thio]triphosphate (ATPγS) or adenosine 5′-(β,γ-imido)triphosphate (AMPPNP). However, the complex was not stable in the absence of nucleotides. These results indicate that ATPγS and AMPPNP repeatedly associated to and dissociated from the complex in a non-cooperative manner. This nucleotide exchange did not induce the dissociation of GroES and substrate from SR398, suggesting the importance of the cooperative dissociation of nucleotides from thecis-ring to release GroES and substrate proteins in the GroEL/GroES reaction cycle.