Stable polymeric nanoballoons: Lyophilization and rehydration of cross-linked liposomes
Stable polymeric nanoballoons: Lyophilization and rehydration of cross-linked liposomes
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DOI：
10.1021/ja0123507
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发表时间：
2002-05-29
影响因子：
15
通讯作者：
O'Brien, DF
中科院分区：
文献类型：
--
作者：
Liu, SC;O'Brien, DF
水合脂质超分子集合体的交联化是稳定这些超分子集合体不受表面活性剂或乙醇破坏的有效方法。非双功能脂类，Acryl/DenPC(16，18)和Sorb/DenPC(18，21)，是一类新的可聚合脂质的例子，旨在创建交联脂结构。通过对脂质体进行冷冻干燥，然后将其在水中基本完全再分散，证明了交联脂质体的强健特性。用准弹性光散射和透射电子显微镜对所得脂质体与原始样品进行了比较。经冷冻干燥的脂质体粉末复水后，其大小和结构均无明显变化。此外，再水化的交联脂质体继续抵抗表面活性物质的增溶。中性交联脂质体在冷冻干燥后，借助浴液超声作用，主要被重新分散。用中性脂质体观察到的少量残留脂质体聚集可以通过在脂质体中加入表面电荷或将亲水性聚合物，例如聚乙二醇附接到脂质体上来防止。
The cross-linking of supramolecular assemblies of hydrated lipids is an effective method to stabilize these assemblies to disruption by surfactants or aqueous alcohol. The heterobifunctional lipids, Acryl/DenPC(16,18) and Sorb/DenPC(18,21), are examples of a new class of polymerizable lipid designed for the creation of cross-linked lipid structures. The robust nature of cross-linked liposomes was demonstrated by lyophilization of the liposomes followed by their essentially complete redispersion in water. The resulting liposomes were compared to the original sample by quasi-elastic light scattering and transmission electron microscopy. There was no major change in the size or structure of the cross-linked liposomes after rehydration of the freeze-dried powder of liposomes. Moreover, the rehydrated cross-linked liposomes continued to be resistant to surfactant solubilization. Neutral cross-linked liposomes were predominantly redispersed after freeze-drying with the aid of bath sonication. The small amount of residual liposome aggregation observed with neutral liposomes could be prevented by incorporating a surface charge into the liposome or attaching hydrophilic polymers, for example, poly(ethylene glycol), onto the liposome.