Ammonium Removal by the Oxygen-Limited Autotrophic Nitrification-Denitrification System
Ammonium Removal by the Oxygen-Limited Autotrophic Nitrification-Denitrification System
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DOI：
10.1128/aem.64.11.4500-4506.1998
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发表时间：
1998-11
影响因子：
4.4
通讯作者：
L. Kuai;W. Verstraete
中科院分区：
文献类型：
--
作者：
L. Kuai;W. Verstraete
摘要本实验室规模的研究揭示了以普通硝化污泥为生物催化剂的限氧自养硝化-反硝化（OLAND）系统在一步去除富氮废水中氮的潜力。在连续间歇式反应器中，处理含有1 g NH 4 +-N升-1和矿物质的合成废水。用pH控制器和计时器双重控制向反应器的氧气供应。在0.13 g NH 4 +-N升-1天-1的容积负荷率（Bv）下，约22%的进料NH 4 +-N转化为NO2--N或NO3--N，38%保留为NH 4 +-N，其余40%主要以N2形式去除。氮的比去除率约为50 mg N升-1天-1，相当于16 mg N g挥发性悬浮固体-1天-1。催化OLAND过程的微生物被认为是以铵氧化菌为主的正常硝化菌。在OLAND系统中，氮的损失被认为是通过NH 4+氧化为N2，NO2−作为电子受体而发生的。羟胺促进NH 4+和NO2−的去除。羟胺氧化还原酶（HAO）或HAO相关酶可能是氮损失的原因。
ABSTRACT The present lab-scale research reveals the potential of implementation of an oxygen-limited autotrophic nitrification-denitrification (OLAND) system with normal nitrifying sludge as the biocatalyst for the removal of nitrogen from nitrogen-rich wastewater in one step. In a sequential batch reactor, synthetic wastewater containing 1 g of NH4+-N liter−1 and minerals was treated. Oxygen supply to the reactor was double-controlled with a pH controller and a timer. At a volumetric loading rate (Bv) of 0.13 g of NH4+-N liter−1 day−1, about 22% of the fed NH4+-N was converted to NO2−-N or NO3−-N, 38% remained as NH4+-N, and the other 40% was removed mainly as N2. The specific removal rate of nitrogen was on the order of 50 mg of N liter−1 day−1, corresponding to 16 mg of N g of volatile suspended solids−1 day−1. The microorganisms which catalyzed the OLAND process are assumed to be normal nitrifiers dominated by ammonium oxidizers. The loss of nitrogen in the OLAND system is presumed to occur via the oxidation of NH4+ to N2 with NO2− as the electron acceptor. Hydroxylamine stimulated the removal of NH4+ and NO2−. Hydroxylamine oxidoreductase (HAO) or an HAO-related enzyme might be responsible for the loss of nitrogen.