Modeling myosin-dependent rearrangement and force generation in an actomyosin network
Modeling myosin-dependent rearrangement and force generation in an actomyosin network
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DOI：
10.1016/j.jtbi.2011.04.004
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发表时间：
2011-07-21
影响因子：
2
通讯作者：
Adachi, Taiji
中科院分区：
文献类型：
--
作者：
Inoue, Yasuhiro;Tsuda, Shunsuke;Adachi, Taiji
肌动球蛋白收缩性是驱动肌动球蛋白网络重排的主要力产生机制；它是细胞重塑和运动等细胞功能的基础。因此，为了阐明细胞功能出现的机械化学基础，了解肌动球蛋白收缩性和肌动球蛋白网络重排之间的关系至关重要。为此，在本研究中，我们提出了一种新的基于颗粒的模型，用于模拟肌动蛋白、非肌肉肌球蛋白 II 和 α-肌动蛋白的运动。为了证实该模型的有效性，我们成功模拟了肌动球蛋白丝的滑动和弯曲运动，这些运动被观察为迁移角膜细胞中肌动球蛋白网络动态重排的基本行为。接下来，我们模拟了肌动球蛋白网络的动态重排。我们的模拟结果表明，除了增加肌动球蛋白丝在网络中产生的力之外，肌球蛋白密度分数的增加还诱导肌动球蛋白丝从网络到束的高阶结构转变。我们将模拟结果与实验结果进行比较，并确认桥接粘着斑的肌动球蛋白束和肌球蛋白依赖性肌球蛋白网络重排的特征与实验观察到的结果定性一致。 (C) 2011 Elsevier Ltd. 保留所有权利。
Actomyosin contractility is a major force-generating mechanism that drives rearrangement of actomyosin networks; it is fundamental to cellular functions such as cellular reshaping and movement. Thus, to clarify the mechanochemical foundation of the emergence of cellular functions, understanding the relationship between actomyosin contractility and rearrangement of actomyosin networks is crucial. For this purpose, in this study, we present a new particulate-based model for simulating the motions of actin, non-muscle myosin II, and alpha-actinin. To confirm the model's validity, we successfully simulated sliding and bending motions of actomyosin filaments, which are observed as fundamental behaviors in dynamic rearrangement of actomyosin networks in migrating keratocytes. Next, we simulated the dynamic rearrangement of actomyosin networks. Our simulation results indicate that an increase in the density fraction of myosin induces a higher-order structural transition of actomyosin filaments from networks to bundles, in addition to increasing the force generated by actomyosin filaments in the network. We compare our simulation results with experimental results and confirm that actomyosin bundles bridging focal adhesions and the characteristics of myosin-dependent rearrangement of actomyosin networks agree qualitatively with those observed experimentally. (C) 2011 Elsevier Ltd. All rights reserved.