IMMUNOLOGICAL STUDIES OF AGING .2. LOSS OF IGG AND HIGH AVIDITY PLAQUE-FORMING CELLS AND INCREASED SUPPRESSOR CELL ACTIVITY IN AGING MICE
IMMUNOLOGICAL STUDIES OF AGING .2. LOSS OF IGG AND HIGH AVIDITY PLAQUE-FORMING CELLS AND INCREASED SUPPRESSOR CELL ACTIVITY IN AGING MICE
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DOI：
10.1084/jem.144.4.1037
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发表时间：
1976-01-01
影响因子：
15.3
通讯作者：
WEKSLER, ME
中科院分区：
文献类型：
--
作者：
GOIDL, EA;INNES, JB;WEKSLER, ME
对二硝基苯化[DNP]牛γ的免疫应答的大小和异质性使用空斑形成细胞抑制[PFC]测定法在细胞水平上测量老年和年轻小鼠的球蛋白[BGG]。24个月的主要和次要反应。与2月龄小鼠相比，老年小鼠对DNP决定簇的亲和力明显降低，古老的动物在免疫时给予细菌[大肠杆菌]脂多糖增加了12和24个月时观察到的异质性限制。老老鼠24个月时，间接PFC较直接PFC受到更严重的抑制。老老鼠同源，致死性辐照，2个月。用老年脾细胞重建的老年小鼠表现出老年小鼠中所见的对DNP-BGG的抑制和限制性反应。当108个年轻胸腺细胞与老年脾细胞一起给予时，反应的异质性增加。当2和24个月。将年老的脾细胞一起转移到年轻的受体中，对年轻的脾细胞的反应程度显著降低。因此，似乎有一个损失的胸腺辅助细胞和抑制活性的增加，在老年动物。
The magnitude and heterogeneity of the immune response to dinitrophenylated [DNP] bovine .gamma. globulin [BGG] was measured in aged and young mice at a cellular level using an inhibition of plaque-forming cell [PFC] assay. The primary and secondary responses of 24 mo. old mice were markedly depressed in magnitude and restricted in avidity for the DNP determinant when compared to 2 mo. old animals. Bacterial [Escherichia coli] lipopolysaccharide given at the time of immunization increased the restriction in heterogeneity seen in 12 and 24 mo. old mice. Indirect PFC were more severely depressed than direct PFC in 24 mo. old mice. Syngeneic, lethally irradiated, 2 mo. old mice reconstituted with aged spleen cells exhibit the depressed and restricted response to DNP-BGG seen in old mice. When 108 young thymus cells were given together with old spleen cells, the heterogeneity of the response was increased. When 2 and 24 mo. old spleen cells were transferred together into young recipients the magnitude of the response to the young spleen cells markedly reduced. Thus, there appears to be a loss of thymic-helper cells and an increase in suppressor activity in aged animals.