Anti-inflammatory effects of an oxylipin-containing lyophilised biomass from a microalga in a murine recurrent colitis model
Anti-inflammatory effects of an oxylipin-containing lyophilised biomass from a microalga in a murine recurrent colitis model
复制标题
DOI：
10.1017/s0007114516004189
复制
发表时间：
2016-12-01
影响因子：
3.6
通讯作者：
Motilva, Virginia
中科院分区：
文献类型：
--
作者：
Avila-Roman, Javier;Talero, Elena;Motilva, Virginia
饮食和营养因素已成为炎症性肠病（IBD）的可能干预措施，其特征是肠粘膜的慢性不受控制的炎症。微藻物种是n-3 PUFA和衍生的氧化脂质的有希望的来源，氧化脂质是在炎症的解决中具有关键作用的脂质介质。本研究的目的是研究来自德巴利衣原体的含有羟脂质的冻干生物质对复发性2，4，6-三硝基苯磺酸（TNBS）诱导的结肠炎小鼠模型的影响。通过每周结肠内滴注低剂量TNBS在BALB/c小鼠中诱导结肠的中度慢性炎症。冻干微藻生物质的施用在结肠炎诱导前2周开始，并在整个结肠炎发展过程中持续。在最后一次TNBS攻击后48小时处死小鼠。口服施用微藻生物质减少了TNBS诱导的肠道炎症，这通过抑制体重减轻、改善结肠形态和降低促炎细胞因子TNF-α、IL-1 β、IL-6和IL-17来证明。该产品还下调结肠诱导型一氧化氮、环氧合酶2和NF-κ B的表达，并增加PPAR-gamma。此外，冻干微藻生物量上调抗氧化转录因子核因子E2相关因子2和靶基因血红素加氧酶1的表达。本研究首次描述了来自C. debaryana在小鼠中复发性TNBS诱导的结肠炎模型的急性期中的作用。这些发现表明，这种微生物制剂或衍生的氧脂素作为营养药物在治疗IBD中的潜在用途。
Diet and nutritional factors have emerged as possible interventions for inflammatory bowel diseases (IBD), which are characterised by chronic uncontrolled inflammation of the intestinal mucosa. Microalgal species are a promising source of n-3 PUFA and derived oxylipins, which are lipid mediators with a key role in the resolution of inflammation. The aim of the present study was to investigate the effects of an oxylipin-containing lyophilised biomass from Chlamydomonas debaryana on a recurrent 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis mice model. Moderate chronic inflammation of the colon was induced in BALB/c mice by weekly intracolonic instillations of low dose of TNBS. Administration of the lyophilised microalgal biomass started 2 weeks before colitis induction and was continued throughout colitis development. Mice were killed 48 h after the last TNBS challenge. Oral administration of the microalgal biomass reduced TNBS-induced intestinal inflammation, evidenced by an inhibition of body weight loss, an improvement in colon morphology and a decrease in pro-inflammatory cytokines TNF-alpha, IL-1 beta, IL-6 and IL-17. This product also down-regulated colonic expressions of inducible nitric oxide, cyclo-oxygenase 2 and NF-kappa B, as well as increased PPAR-gamma. In addition, lyophilised microalgal biomass up-regulated the expressions of the antioxidant transcription factor nuclear factor E2-related factor 2 and the target gene heme oxygenase 1. This study describes for the first time the prophylactic effects of an oxylipin-containing lyophilised microalgae biomass from C. debaryana in the acute phase of a recurrent TNBS-induced colitis model in mice. These findings suggest the potential use of this microalga, or derived oxylipins, as a nutraceutical in the treatment of IBD.