Effect of exercise training on in vivo lipolysis in intra-abdominal adipose tissue in rats
Effect of exercise training on in vivo lipolysis in intra-abdominal adipose tissue in rats
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DOI：
10.1152/ajpendo.2000.279.3.e585
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发表时间：
2000-09-01
影响因子：
5.1
通讯作者：
Galbo, H
中科院分区：
文献类型：
--
作者：
Enevoldsen, LH;Stallknecht, B;Galbo, H
腹内肥胖与心血管疾病和非胰岛素依赖型糖尿病有关，体育锻炼被认为可以缓解这些疾病。我们比较了肾上腺素刺激的体内三种腹腔内脂肪组织（ATs：腹膜后、参数和肠系膜）和皮下AT的脂肪分解，我们还研究了体育训练的效果。此外，我们在体内研究了体育训练对肾上腺素刺激的肌肉脂肪分解的影响。雌性大鼠接受游泳训练（15周，n = 8）或久坐（n = 7）。麻醉下，两阶段静脉输注肾上腺素（60分钟，剂量分别为80和200 ng）。公斤(1)。分别取Min(-1)，并用微透析技术测定局部间质甘油浓度。用微球测量血流量。训练增加了所有ATs的血流量[平均：73 +/- 12（训练）vs. 14 +/- 4（久坐）)ml.100g（-1）。min(-1)， P< 0.05；然而，肾上腺素刺激的间质甘油浓度增加或不变。在训练和不运动的大鼠中，腹腔内的间质甘油浓度都高于皮下AT。在骨骼肌中，训练大鼠和不运动大鼠间质甘油浓度和血流量没有差异。综上所述，体内脂肪分解在基础状态和腹腔内肾上腺素刺激下均高于皮下AT，训练可能通过增强腹腔内脂肪库的脂肪分解来减轻腹腔内肥胖。
Intra-abdominal obesity is associated with cardiovascular disease and noninsulin-dependent diabetes mellitus, and physical training has been suggested to alleviate these conditions. We compared epinephrine-stimulated lipolysis in vivo in three intra-abdominal adipose tissues (ATs: retroperitoneal, parametrial, and mesenteric) and in subcutaneous AT, and we also studied the effect of physical training. Moreover, we studied the effect of physical training on epinephrine-stimulated lipolysis in muscle in vivo. Female rats were either swim trained (15 wk, n = 8) or sedentary (n = 7). Under anesthesia, a two-stage intravenous epinephrine infusion (60 min of 80 and 200 ng . kg(-1).min(-1), respectively) was carried out, and local interstitial glycerol concentration was measured by the microdialysis technique. Blood flow was measured by microspheres. Training increased blood flow in all ATs [on average: 73 +/- 12 (trained) vs. 14 +/- 4 (sedentary) ml.100g(-1).min(-1), P< 0.05]; nevertheless, epinephrine-stimulated interstitial glycerol concentrations were increased or unchanged. Interstitial glycerol concentration was higher in intra-abdominal than in subcutaneous AT in both trained and sedentary rats. In skeletal muscle, interstitial glycerol concentration and blood flow did not differ between trained and sedentary rats. In conclusion, in vivo lipolysis is higher both in the basal state and during epinephrine-stimulation in intraabdominal than in subcutaneous AT, and training may be beneficial in alleviating intra-abdominal obesity by enhancing lipolysis in intra-abdominal fat depots.