Conservative segregation of nucleosome core histones
Conservative segregation of nucleosome core histones
复制标题
核小体核心组蛋白的保守分离
作者：
I. Leffak
密度标记的研究表明，新生组蛋白不与父母组蛋白在核小体cores 1，2组装。然而，在其他实验室的实验，检查组蛋白沉积相对于新合成的DNA，已被解释为表明，相当大比例的核心组蛋白（>15%）是随机在每个染色质复制3 -8。这里提供的数据支持我们以前的结果，表明保守组装的核小体核心组蛋白八聚体在连续的细胞世代中保守分离。还表明，在1-β-D-阿拉伯呋喃糖基胞嘧啶抑制DNA合成期间组装的核小体核心保守分离至少5或6代细胞。这些结果表明，核小体核心的非随机组装不仅仅是一个巧合的组蛋白运输到细胞核的机制，核小体分离的保守模式是染色质复制的一个基本特征，这是稳定的染色质包装的调制。
Density labelling studies have shown that nascent histones are not mixed with parental histones during the assembly of nucleosome cores1,2. However, experiments in other laboratories, examining histone deposition with respect to newly synthesized DNA, have been interpreted as suggesting that a substantial proportion of core histones (>15%) are randomized at each chromatin replication3–8. The data presented here support our previous results in showing that conservatively assembled nucleosome core histone octamers are conservatively segregated over successive cell generations. It is also shown that the nucleosome cores assembled during 1-β-D-arabinofuranosylcytosine inhibition of DNA synthesis are conservatively segregated for a minimum of five or six cell generations. These results suggest that the nonrandom assembly of nucleosome cores is not merely a coincidence of the mechanism of histone transport into the nucleus and that the conservative mode of nucleosome segregation is a fundamental feature of chromatin replication, one which is stable to modulations in chromatin packaging.