New continuous-flow analysis for simultaneous determination of creatinine and uric acid in 200 microliters of serum without use of a dialyzer.
New continuous-flow analysis for simultaneous determination of creatinine and uric acid in 200 microliters of serum without use of a dialyzer.
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新型连续流分析无需使用透析器即可同时测定 200 微升血清中的肌酐和尿酸。
DOI：
10.1093/clinchem/24.9.1578
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发表时间：
1978
影响因子：
9.3
通讯作者：
A. Wongvibulsin
中科院分区：
文献类型：
--
作者：
P. Lolekha;V. Chantarothipol;A. Wongvibulsin
我们提出了一种直接连续流（AutoAnalyzer II）测定血清肌酐和尿酸的新方法。该歧管结构简单，价格低廉，可以在实验室中构建。只需要200微升血清；分析速度为每小时60个样品。十二烷基硫酸钠的掺入和同时提供空白减法使省略透析步骤成为可能。我们的方法不需要线性化器，因为仪器响应和肌酐和尿酸浓度分别与200和120 mg/l线性相关。稳态百分比、相互作用百分比和回收率均可接受，精密度好，所得结果与比较方法的结果吻合良好。干扰很少，当遇到干扰时，通常比改进的Technicon方法要小。明显溶血只干扰尿酸测定；明显浑浊对肌酐检测结果无影响。总胆红素为50 mg/l和100 mg/l的黄疸血清分别对肌酐和尿酸的测定结果有显著干扰。
We present a new method for direct continuous-flow (AutoAnalyzer II) measurement of serum creatinine and uric acid. The manifold is simple, inexpensive, and can be constructed in the laboratory. Only 200 microliters of serum is needed; analysis rate is 60 samples per hour. The incorporation of sodium dodecyl sulfate and the simultaneous provision of blank subtraction make it possible to omit the dialysis step. Our method does not require the linearizer, since instrument response and concentration of creatinine and uric acid are linearly related to 200 and 120 mg/liter, respectively. The percentages of steady state, interaction, and recovery are acceptable, Precision is excellent and the results obtained from the new method correlate well with those obtained by the comparison methods. Interferences are few and, when encountered, are generally smaller than in the modified Technicon method. Marked hemolysis interferes only with the uric acid assay; marked turbidity has no effect on results for creatinine. Icteric serum with total bilirubin of 50 and 100 mg/liter interferes significantly with results for creatinine and uric acid, respectively, by the new method.