Inhibitory effects of fungal bis(naphtho-γ-pyrone) derivatives on nitric oxide production by a murine macrophage-like cell line, RAW 264.7, activated by lipopolysaccharide and interferon-γ
Inhibitory effects of fungal bis(naphtho-γ-pyrone) derivatives on nitric oxide production by a murine macrophage-like cell line, RAW 264.7, activated by lipopolysaccharide and interferon-γ
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DOI：
10.1248/bpb.28.786
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发表时间：
2005-05-01
影响因子：
2
通讯作者：
Kitanaka, S
中科院分区：
文献类型：
--
作者：
Ishii, R;Horie, M;Kitanaka, S
研究了6种真菌双（萘并-γ-吡喃酮）衍生物对脂多糖和干扰素-γ激活的小鼠巨噬细胞样细胞系RAW 264.7产生一氧化氮（NO）的抑制作用。在这些衍生物中，毛色素（4）（IC 50：0.8 μ M）、头孢色素（1）（IC 50：1.5 μ M）和二氢异茅斯菌素A（6）（IC 50：2.8 μ M）表现出强的抑制活性。双（萘并-γ-吡喃酮）衍生物不影响诱导型一氧化氮合酶（iNOS）的酶活性。然而，这些衍生物显着降低诱导的iNOS蛋白和iNOS mRNA的表达。这些结果表明，双（萘并-γ-吡喃酮）衍生物具有抑制活化的巨噬细胞产生NO的药理能力。
Inhibitory effects of six fungal bis(naphtho-gamma-pyrone) derivatives on nitric oxide (NO) production by a murine macrophage-like cell line, RAW 264.7, which was activated by lipopolysaccharide and interferon-gamma were examined. Among these derivatives, chaetochromin (4) (IC50: 0.8 mu M), cephalochromin (1) (IC50 1.5 mu M), and dihydroisoustilaginoidin A (6) (IC50 2.8 mu M) exhibited strong inhibitory activity. The bis(naphtho-gamma-pyrone) derivatives did not affect the enzyme activity of inducible nitric oxide synthase (iNOS). However, these derivatives significantly reduced both the induction of iNOS protein and iNOS mRNA expression. These results suggest that the bis(naphtho-gamma-pyrone) derivatives have the pharmacologic ability to suppress NO production by activated macrophages.