Comparison of progenitor cell concentration techniques: continuous flow separation versus density-gradient isolation.
Comparison of progenitor cell concentration techniques: continuous flow separation versus density-gradient isolation.
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祖细胞浓缩技术的比较：连续流分离与密度梯度分离。
DOI：
10.1089/scd.1.1993.2.315
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
S. Noga
中科院分区：
文献类型：
--
作者：
Davis Jm;Schepers Kg;L. Eby;S. Noga
使用两种技术处理骨髓收获物;在COBE Spectra上制备20个单核细胞浓度（MNC），并与使用COBE 2991分离的10个轻密度细胞组分进行比较。两种方法回收的有核细胞百分比基本相同（22%对21%），MNC和光密度产物中CFU-GM祖细胞回收率分别为132%和100%，CD 34+细胞回收率分别为101%和104%。Spectra MNC浓缩液的优势在于其采用美国食品药品监督管理局批准用于注射的试剂制备。然而，MNC浓缩物的平均红细胞压积为4%，而在低密度细胞组分中无法测量。只有当骨髓需要用4-氢过氧环磷酰胺净化或红细胞干扰处理时，这种差异才显著。光谱程序回收了更大比例的MNC细胞，并有更少的污染与成熟粒细胞比密度梯度技术。当红细胞不影响骨髓处理方案时，Spectra MNC浓缩液是轻密度细胞制剂的安全替代品。
Bone marrow harvests were processed using two techniques; 20 mononuclear cell concentrations (MNC) were prepared on the COBE Spectra and compared to 10 light-density cell fractions isolated using the COBE 2991. Both procedures recovered essentially the same percentage of nucleated cells (22 versus 21%) and gave progenitor cell recoveries of 132 and 100% of the CFU-GM and 101 and 104% of the CD34+ cells in the MNC and light-density products, respectively. The advantage of the Spectra MNC concentrate is that it was prepared with reagents approved for injection by the Food and Drug Administration. However, the average hematocrit on the MNC concentrate was 4%, while it was unmeasurable in the light-density cell fractions. This difference is significant only when bone marrows require purging with 4-hydroperoxycyclophosphamide or erythrocytes otherwise interfere with processing. The Spectra procedure recovered a larger percentage of MNC cells and had less contamination with mature granulocytes than did the density-gradient technique. When erythrocytes do not affect the bone marrow processing protocol, a Spectra MNC concentrate is a safe substitute for a light-density cell preparation.
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自体骨髓移植临床净化过程中 4-氢过氧环磷酰胺活性的变化。
影响因子：
20.3
作者：
Jones,RJ;Zuehlsdorf,M;Rowley,SD;Hilton,J;Santos,GW;Sensenbrenner,LL;Colvin,OM
通讯作者：
Colvin,OM
急性白血病自体骨髓移植：用 4-氢过氧环磷酰胺体外治疗骨髓清除肿瘤细胞的 I 期研究。
影响因子：
20.3
作者：
Kaizer,H;Stuart,RK;Brookmeyer,R;Beschorner,WE;Braine,HG;Burns,WH;Fuller,DJ;Korbling,M;Mangan,KF;Saral,R
通讯作者：
Saral,R
影响因子：
20.3
作者：
Davis Jm;S. Rowley;H. Braine;S. Piantadosi;G. Santos
通讯作者：
Davis Jm;S. Rowley;H. Braine;S. Piantadosi;G. Santos
影响因子：
20.3
作者：
R. Champlin;W. Ho;J. Gajewski;S. Feig;M. Burnison;G. Holley;P. Greenberg;Kyoung Lee;I. Schmid;J. Giorgi
通讯作者：
R. Champlin;W. Ho;J. Gajewski;S. Feig;M. Burnison;G. Holley;P. Greenberg;Kyoung Lee;I. Schmid;J. Giorgi
自体骨髓移植联合药物清除的I期研究。
DOI：
10.1200/jco.1991.9.12.2210
发表时间：
1991
期刊：
Journal of clinical oncology : official journal of the American Society of Clinical Oncology
影响因子：
--
作者：
Rowley,SD;Miller,CB;Piantadosi,S;Davis,JM;Santos,GW;Jones,RJ
通讯作者：
Jones,RJ