Acoustically Mounted Microcrystals Yield High-Resolution X-ray Structures
Acoustically Mounted Microcrystals Yield High-Resolution X-ray Structures
复制标题
DOI：
10.1021/bi200549x
复制
发表时间：
2011-05-31
期刊：
影响因子：
2.9
通讯作者：
Orville, Allen M.
中科院分区：
文献类型：
--
作者：
Soares, Alexei S.;Engel, Matthew A.;Orville, Allen M.
我们展示了一个一般性的战略，确定从淋浴的微晶结构。它使用声学液滴喷射将2.5 nL液滴从微晶浆料表面通过空气转移到安装微网针上。通过光栅扫描几微米的X射线束穿过低温冷却的微网来定位单个微晶。从几个微米大小的晶体合并的X射线衍射数据集用于确定1.8埃分辨率的晶体结构。
We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micrometer X-ray beam across the cryocooled micromeshes. X-ray diffraction data sets merged from several micrometer-sized crystals are used to determine 1.8 angstrom resolution crystal structures.