The role of the ERK1/2 signalling pathway in the pathogenesis of female stress urinary incontinence
The role of the ERK1/2 signalling pathway in the pathogenesis of female stress urinary incontinence
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DOI：
10.1177/0300060513493995
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发表时间：
2013-08-01
影响因子：
1.6
通讯作者：
Zhou, Yong
中科院分区：
文献类型：
--
作者：
Huang, Qiong;Jin, Hangmei;Zhou, Yong
目的：目的：探讨细胞外调节蛋白激酶（ERK）1/2和磷酸化（p）-ERK 1/2在女性压力性尿失禁（SUI）发病中的作用。对p-ERK 1/2进行免疫组织化学和Western印迹。原代阴道成纤维细胞培养物中孵育的存在或不存在的PD 98059（ERK激酶的抑制剂）和胶原蛋白I和III的mRNA和蛋白的水平进行了检查，通过定量逆转录聚合酶链反应和Western blot，respectively.Results：p-ERK 1/2的水平显着降低阴道壁组织从SUI患者（n=10）与对照组（n = 10）相比。PD 98059治疗显着降低胶原蛋白I和III的mRNA和protein.Conclusions水平：女性SUI与p-ERK 1/2水平降低相比，与对照组，和ERK 1/2信号通路的抑制抑制胶原蛋白I型和III型合成在阴道壁成纤维细胞。
Objective: To investigate the role of extracellular-regulated protein kinase (ERK)1/2 and phosphorylated (p)-ERK1/2 in the pathogenesis of female stress urinary incontinence (SUI).Methods: Anterior vaginal wall tissue was collected from women with SUI and control subjects. Immunohistochemistry and Western blotting were performed for p-ERK1/2. Primary vaginal fibroblast cultures were incubated in the presence or absence of PD98059 (an inhibitor of ERK kinase) and levels of collagen I and III mRNA and protein were examined by quantitative reverse transcription-polymerase chain reaction and Western blot, respectively.Results: Levels of p-ERK1/2 were significantly lower in vaginal wall tissue from patients with SUI (n=10) compared with controls (n=10). PD98059 treatment significantly reduced levels of collagen I and III mRNA and protein.Conclusions: Female SUI is associated with reduced levels of p-ERK1/2 compared with controls, and inhibition of the ERK1/2 signalling pathway inhibits collagen type I and III synthesis in vaginal wall fibroblasts.