Abscisic acid ameliorates atherosclerosis by suppressing macrophage and CD4+ T cell recruitment into the aortic wall.
Abscisic acid ameliorates atherosclerosis by suppressing macrophage and CD4+ T cell recruitment into the aortic wall.
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DOI：
10.1016/j.jnutbio.2009.10.003
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发表时间：
2010-12
影响因子：
5.6
通讯作者：
Bassaganya-Riera, Josep
中科院分区：
文献类型：
--
作者：
Guri, Amir J.;Misyak, Sarah A.;Hontecillas, Raquel;Hasty, Alyssa;Liu, Dongmin;Si, Hongwei;Bassaganya-Riera, Josep
脱落酸（ABA）是一种天然的植物激素，当添加到肥胖小鼠的饮食中时，可以改善胰岛素敏感性并减少脂肪组织炎症。本研究的目的是探讨脱落酸（ABA）预防或改善动脉粥样硬化的机制。载脂蛋白e缺乏（ApoE−/−）小鼠分别饲喂含或不含ABA的高脂饲料84天。在第0、28、56和72天评估收缩压。对主动脉根壁的基因表达、免疫细胞浸润和组织学病变进行了评估。采用体外培养的人主动脉内皮细胞，研究了ABA对3′，5′-环腺苷单磷酸（cAMP）和一氧化氮（NO）生成的影响。我们报告说，aba处理小鼠的收缩压显著改善，并减少F4/80+CD11b+巨噬细胞和CD4+ T细胞在主动脉根壁的积累。在分子水平上，ABA显著增强主动脉内皮一氧化氮合酶（eNOS）活性，并有抑制主动脉血管细胞粘附分子-1 （VCAM-1）和单核细胞趋化蛋白-1 （MCP-1）表达和血浆MCP-1浓度的趋势。ABA还导致人主动脉内皮细胞内cAMP和NO浓度呈剂量依赖性增加，eNOS mRNA表达上调。这是首次报道表明ABA可以预防或改善ApoE−/−小鼠动脉粥样硬化诱导的高血压、免疫细胞向主动脉根壁募集以及上调主动脉eNOS表达。
Abscisic acid (ABA) is a natural phytohormone which improves insulin sensitivity and reduces adipose tissue inflammation when supplemented into diets of obese mice. The objective of this study was to investigate the mechanisms by which abscisic acid (ABA) prevents or ameliorates atherosclerosis. Apolipoprotein E-deficient (ApoE −/−) mice were fed high-fat diets with or without ABA for 84 days. Systolic blood pressure was assessed on days 0, 28, 56, and 72. Gene expression, immune cell infiltration, and histological lesions were evaluated in the aortic root wall. Human aortic endothelial cells were used to examine the effect of ABA on 3’, 5’-cyclic adenosine monophosphate (cAMP) and nitric oxide (NO) production in vitro. We report that ABA-treated mice had significantly improved systolic blood pressure and decreased accumulation of F4/80+CD11b+ macrophages and CD4+ T cells in aortic root walls. At the molecular level, ABA significantly enhanced aortic endothelial nitric oxide synthase (eNOS) and tended to suppress aortic vascular cell adhesion molecule-1 (VCAM-1) and monocyte chemoattractant protein-1 (MCP-1) expression and plasma MCP-1 concentrations. ABA also caused a dose-dependent increase in intracellular concentrations of cAMP and NO and upregulated eNOS mRNA expression in human aortic endothelial cells. This is the first report showing that ABA prevents or ameliorates atherosclerosis-induced hypertension, immune cell recruitment into the aortic root wall, and upregulates aortic eNOS expression in ApoE−/− mice.
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影响因子：
6.3
作者：
Guri, Amir J.;Hontecillas, Raquel;Bassaganya-Riera, Josep
通讯作者：
Bassaganya-Riera, Josep
DOI：
10.1161/01.atv.14.1.133
发表时间：
1994-01-01
期刊：
ARTERIOSCLEROSIS AND THROMBOSIS
影响因子：
--
作者：
NAKASHIMA, Y;PLUMP, AS;ROSS, R
通讯作者：
ROSS, R
影响因子：
64.8
作者：
Pascual, G;Fong, AL;Glass, CK
通讯作者：
Glass, CK
影响因子：
4.3
作者：
Bassaganya-Riera, Josep;Misyak, Sarah;Hontecillas, Raquel
通讯作者：
Hontecillas, Raquel
影响因子：
82.9
作者：
Indolfi, C;Avvedimento, EV;Chiarello, M
通讯作者：
Chiarello, M