The cryoloop: an adaptable reversible cooling deactivation method for behavioral or electrophysiological assessment of neural function
The cryoloop: an adaptable reversible cooling deactivation method for behavioral or electrophysiological assessment of neural function
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DOI：
10.1016/s0165-0270(98)00165-4
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发表时间：
1999-01-01
影响因子：
3
通讯作者：
Horel, JA
中科院分区：
文献类型：
--
作者：
Lomber, SG;Payne, BR;Horel, JA
我们描述了一种非常适应性强的可逆失活技术，用于神经回路的行为或电生理学分析。 Cryoloop 装置可以永久植入或局部应用于急性制剂中，以对中枢神经系统（例如大脑皮层或中脑）的离散表面区域施加冷量。 Cryoloop 由定制形状的不锈钢皮下注射管组成，通过使冷冻甲醇流过管腔来实现冷却。 Cryoloop 温度由连接到回路接头上的微热电偶进行监控，并且可以保持在所需温度的 +/- 1 摄氏度范围内。在长期制剂中，植入的冷冻环已在猫和猴子体内保存了超过 2 年。在此期间之后，失活组织中不再发生结构、代谢或功能变化，并且保持冷却诱导效应的完全可逆性。多个冷冻探针的操作为实验方案提供了极大的灵活性，允许进行双功能和三功能解离，并大大加强了实验设计。 (C) 1999 Elsevier Science B.V. 保留所有权利。
We describe a very adaptable reversible inactivation technique for the behavioral or electrophysiological analysis of neural circuits. The cryoloop device can be permanently implanted or topically applied in an acute preparation to apply cold to discrete surface regions of the central nervous system (e.g. cerebral cortex or midbrain). The cryoloop consists of a custom shaped, stainless steel, hypodermic tubing and cooling is effected by passing chilled methanol through the lumen of the tubing. Cryoloop temperature is monitored by a microthermocouple attached to the union of the loop, and can be maintained within +/- 1 degrees C of a desired temperature. In chronic preparations, implanted cryoloops have been maintained in cats and monkeys for periods in excess of 2 years. After this period there are no structural, metabolic of functional changes in the deactivated tissue, and full reversibility of cooling-induced effects is maintained. Operation of multiple cryoprobes provides great flexibility of experimental protocols, permits double and triple functional dissociations to be made, and strengthens experimental design considerably. (C) 1999 Elsevier Science B.V. All rights reserved.