Bilirubin inhibits the TNFα-related induction of three endothelial adhesion molecules
Bilirubin inhibits the TNFα-related induction of three endothelial adhesion molecules
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DOI：
10.1016/j.bbrc.2009.06.029
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发表时间：
2009-08-21
影响因子：
3.1
通讯作者：
Tiribelli, Claudio
中科院分区：
文献类型：
--
作者：
Mazzone, Graciela Lujan;Rigato, Igino;Tiribelli, Claudio
由于血清非结合胆红素（UCB）水平升高被认为是对抗动脉粥样硬化疾病的独立保护因素，因此我们研究了基于这种效应的分子事件。用 TNF α 和 UCB 处理 HUVEC 和 H5V 细胞，并评估对 E-选择素、VCAM-1 和 ICAM-1 的影响。在 HUVEC 细胞中，UCB 减弱了 TNF α 诱导的 E-选择素 VCAM-1 和 ICAM-1 基因上调。除 ICAM-1 外，在 H5V 细胞中观察到相同的模式。 UCB 还抑制 HUVEC H5V 细胞中的 PMN 内皮粘附。 Western blot 和 FACS 分析证实，UCB 可以阻止 TNF α 诱导的 H5V 细胞中粘附分子蛋白的过度表达。这些数据有助于进一步解释胆红素对动脉粥样硬化发展的保护作用。 (C) 2009 Elsevier Inc. 保留所有权利。
Since an increased serum unconjugated bilirubin (UCB) level has been proposed as an independent protective factor against atherosclerotic disease, we investigated the molecular events at the basis of this effect. HUVEC and H5V cells were treated with TNF alpha and UCB and the effects assessed on E-selectin, VCAM-1 and ICAM-1. In HUVEC cells, UCB blunted the TNF alpha-induced gene upregulation of E-selectin VCAM-1 and ICAM-1. The same pattern was observed in H5V cells except for ICAM-1. UCB also inhibited the PMN endothelial adhesion in HUVEC H5V cells. Western blot and FACS analysis confirmed that UCB prevented TNF alpha-induced over-expression of adhesion molecules proteins in H5V cells. These data contribute to further explain the protective effect of bilirubin against development of atherosclerosis. (C) 2009 Elsevier Inc. All rights reserved.