Expression of Reg/PAP family members during motor nerve regeneration in rat
Expression of Reg/PAP family members during motor nerve regeneration in rat
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DOI：
10.1016/j.bbrc.2005.04.105
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发表时间：
2005-06-24
影响因子：
3.1
通讯作者：
Kiyama, H
中科院分区：
文献类型：
--
作者：
Namikawa, K;Fukushima, M;Kiyama, H
在这项研究中，我们研究了再生基因（Reg）/胰腺炎相关蛋白（PAP）家族成员在大鼠舌下神经损伤后的mRNA表达。除了已鉴定的四个大鼠家族成员（RegI、Reg-2/PAP I、PAP II和PAP III）外，我们新克隆并测序了大鼠IV型Reg基因。在这五个家族成员中，Reg-2/PAP I mRNA的表达在轴突切断后受损的运动神经元中显著增强。此外，在损伤神经的远端部分观察到PAP III mRNA的显著诱导。针对PAP III提出了多克隆抗体，并且使用该抗体的Western印迹分析证实了损伤神经中PAP III蛋白水平的增加。这些结果表明，Reg家族成员可能是受损神经元和胶质细胞之间的新介质，并可能在神经再生过程中发挥关键作用。(c)2005年爱思唯尔公司All rights reserved.
In this study, we examined the expression of mRNAs for Regenerating gene (Reg)/pancreatitis-associated protein (PAP) family members following hypoglossal nerve injury in rats. In addition to four rat family members (RegI, Reg-2/PAP I, PAP II, and PAP III) that had been identified, we newly cloned and sequenced a type-IV Reg gene in rats. Among these five family members, the expression of Reg-2/PAP I mRNA was predominantly enhanced in injured motor neurons after axotomy. Furthermore, a marked induction of PAP III mRNA was observed in the distal part of the injured nerve. A polyclonal antibody was raised against PAP III, and a Western blotting analysis using this antibody confirmed an increased level of PAP III protein in the injured nerve. These results suggest that Reg family members would be new mediators among injured neurons and glial cells, and may play pivotal roles during nerve regeneration. (c) 2005 Elsevier Inc. All rights reserved.