Diel changes in the expression of long wavelength-sensitive and ultraviolet-sensitive opsin genes in the Japanese firefly, Luciola cruciata
Diel changes in the expression of long wavelength-sensitive and ultraviolet-sensitive opsin genes in the Japanese firefly, Luciola cruciata
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DOI：
10.1016/j.gene.2009.02.001
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发表时间：
2009-05-01
期刊：
影响因子：
3.5
通讯作者：
Kainuma, Takahiko
中科院分区：
文献类型：
--
作者：
Oba, Yuichi;Kainuma, Takahiko
雄性和雌性萤火虫之间的性交流涉及生物发光的视觉检测。在本研究中，我们分离出两种不同类型的视蛋白cDNA从日本萤火虫，Luciola cruciata的成年。系统发育分析表明，这些基因对应于长波长敏感和紫外敏感的视蛋白。这与先前的研究结果一致，其中L。cruciata眼显示两个峰，UV和长波长，并且后者基本上匹配λ max=560 nm的生物发光光谱。通过定量PCR分析确定两种视蛋白mRNA水平的昼夜变化。在成年雌性中，长波长敏感视蛋白的mRNA水平在夜间高于白天，并在发光行为最活跃的时间20：00达到峰值。另一方面，紫外线敏感视蛋白的表达水平在白天没有显著变化。在成年男性中，这两种视蛋白表达的昼夜变化并不显著。研究结果表明，雌性十字绣线菊“生物发光敏感”视蛋白的表达水平与其交配行为有关。(C)2009爱思唯尔有限公司版权所有。
Sexual communication between male and female fireflies involves the visual detection of bioluminescence. In the present study, we isolated two different types of opsin cDNAs from an adult of the Japanese firefly, Luciola cruciata. Phylogenetic analysis indicated that these genes correspond to long wavelength-sensitive and ultraviolet-sensitive opsins. This is in agreement with the prior findings, in which the spectral sensitivity of the L. cruciata eye showed two peaks, UV and long wavelength, and the latter substantially matched the bioluminescent spectrum of lambda max=560 nm. Diel changes in both opsins mRNA levels were determined by quantitative PCR analysis. In adult females, the mRNA level of long wavelength-sensitive opsin was higher at night than in the day, and peaked at 20:00, the time when the luminescence behavior was most active. On the other hand, the expression level of ultraviolet-sensitive opsin was not significantly changed during the day. In adult males, diel changes in the expression of both opsins were not significant. The results suggest that the expression level of "bioluminescence-sensitive" opsins in female L cruciata is linked to their mating behavior. (C) 2009 Elsevier B.V. All rights reserved.