Differential expression of DNA polymerase epsilon in resting and activated B lymphocytes is consistent with an in vivo role in replication and not repair.
Differential expression of DNA polymerase epsilon in resting and activated B lymphocytes is consistent with an in vivo role in replication and not repair.
复制标题
DNA聚合酶ε在静息和活化B淋巴细胞中的差异表达与体内复制而非修复作用一致。
DOI：
10.1016/s0161-5890(00)00040-7
复制
发表时间：
2000
影响因子：
3.6
通讯作者：
Gearhart,PJ
中科院分区：
文献类型：
--
作者：
Winter,DB;Phung,QH;Wood,RD;Gearhart,PJ
DNA polymerases may be differentially expressed by cells during periods of quiescence and proliferation. Murine B cells are an ideal population to study because their division time varies widely in vivo, and different subsets can be easily isolated. Consequently, we analyzed RNA from resting cells (B220+peanut agglutinin−) and activated germinal center cells (B220+peanut agglutinin+) from spleens by reverse transcriptase-PCR using primers for five nuclear polymerases and their associated subunits. Gel analyses of the amplified products showed that the rapidly-dividing germinal center B cells expressed DNA polymerases α, β, δ, ϵ, and ζ. The resting B cells did not express polymerases α or ϵ at detectable levels, although they did express polymerases β, δ, and ζ. Thus, polymerase ϵ, as well as α, appears to have a primary role in chromosomal replication of murine B lymphocytes. Further, the lack of expression of polymerase ϵ in resting cells indicates that this enzyme is not used in any DNA repair pathways by these cells. The expression of polymerase ζ by resting cells suggests that it has another role in DNA repair, perhaps recombination, in addition to its function of bypassing damage during chromosomal replication.
