Lactobionic acid enhances the synergistic effect of nisin and thymol against Listeria monocytogenes Scott A in tryptic soy broth and milk
Lactobionic acid enhances the synergistic effect of nisin and thymol against Listeria monocytogenes Scott A in tryptic soy broth and milk
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DOI：
10.1016/j.ijfoodmicro.2017.08.013
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发表时间：
2017-11-02
影响因子：
5.4
通讯作者：
Zhong, Qixin
中科院分区：
文献类型：
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作者：
Chen, Huaiqiong;Zhong, Qixin
单核细胞增生李斯特菌是一种革兰氏阳性的人类机会致病菌，它仍然是食源性疾病的重要原因。人们已经研究了多种天然和合成化合物来抑制L.食品中的单核细胞增多症。具有协同抗菌特性的抗菌剂组合可以减少每种抗菌剂的剂量，这可以通过螯合化合物进一步增强。因此，本研究的目的是确定二元或三元组合的乳糖酸（LBA），乳链菌肽，和百里酚胰蛋白酶大豆肉汤（TSB），2%的低脂牛奶，和全脂牛奶的抗糖尿病特性。结果表明，乳链菌肽、百里酚和LBA的最低抑菌浓度（MIC）分别为125 IU/mL、0.25 mg/mL和10 mg/mL。三元组合在降低抗菌剂的MIC方面最有效，乳链菌肽、百里酚和LBA的MIC分别为31.25 IU/mL、0.0625 mg/mL和1.25 mg/mL。在含0.6%酵母膏的TSB培养基中，L.单核细胞增多症在32 ℃下24小时内在31.25 IU/mL乳链菌肽、0.0625 mg/mL百里酚和1.25 mg/mL LBA的单独或二元抗微生物处理中生长，而其被三元组合完全抑制。在21 ℃下的2%脱脂乳中，分别浓度为250 IU/mL的乳链菌肽、百里酚和LBA的三元组合，2 mg/mL和10 mg/mL的组合在72 h内完全抑制细菌至检测限以下，而120 h后在所有二元组合中仍检测到> 2 log（CFU/mL）的细菌。在全脂牛奶中，500 IU/mL乳链菌肽、2 mg/mL百里酚和10 mg/mL LBA的组合在21 ℃下4小时内将细菌减少至约2 log（CFU/mL），5 d后未观察到细菌恢复。本研究表明乳链菌肽、百里酚和LBA的三元组合物在食品防腐方面具有潜在的应用价值。
Listeria monocytogenes is a Gram-positive opportunistic human pathogen and it remains a significant cause of foodborne illnesses. A variety of natural and synthetic compounds have been studied to inhibit the growth of L. monocytogenes in foods. Antimicrobial combinations with synergistic antilisterial properties can reduce the dose of each antimicrobial, which can be further enhanced by chelating compounds. Therefore, the objective of this study was to determine antilisterial properties of binary or ternary combinations of lactobionic acid (LBA), nisin, and thymol in tryptic soy broth (TSB), 2% reduced-fat milk, and whole milk. The results showed that the minimum inhibitory concentration (MIC) of nisin, thymol and LBA was 125 IU/mL, 0.25 mg/mL, and 10 mg/mL, respectively. The ternary combination was the most effective in reducing MICs of antimicrobials, with the MIC of nisin, thymol, and LBA being 31.25 IU/mL, 0.0625 mg/mL, and 1.25 mg/mL, respectively. In TSB with 0.6% yeast extract, L. monocytogenes grew in individual or binary antimicrobial treatments of 31.25 IU/mL nisin, 0.0625 mg/mL thymol, and 1.25 mg/mL LBA within 24 h at 32 degrees C, while it was completely inhibited by the ternary combination, In 2% reduced-fat milk at 21 degrees C, the ternary combination of nisin, thymol, and LBA at respective concentrations of 250 IU/mL, 2 mg/mL, and 10 mg/mL completely inhibited the bacterium to below the detection limit in 72 h while > 2 log (CFU/mL) bacteria was still detected in all the binary combinations after 120 h. In whole milk, the combination of 500 IU/mL nisin, 2 mg/mL thymol, and 10 mg/mL LBA reduced bacteria to around 2 log (CFU/mL) in 4 h at 21 degrees C, and no bacterial recovery was observed after 5 d. This study suggested the potential of the ternary combination of nisin, thymol and LBA for food preservation.