Salivary mucin: a factor in the lower prevalence of gastroesophageal reflux disease in African-Americans?
Salivary mucin: a factor in the lower prevalence of gastroesophageal reflux disease in African-Americans?
复制标题
唾液粘蛋白：非洲裔美国人胃食管反流病患病率较低的一个因素？
DOI：
10.1111/j.1572-0241.2000.03253.x
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Troxler,RF
中科院分区：
文献类型：
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作者：
Rayment,SA;Liu,B;Offner,GD;Oppenheim,FG;Troxler,RF
目的：唾液的有机和无机成分被认为是食管的保护成分，不同种族中一种或多种这些成分的缺乏可能是胃食管反流病（GERD）患病率的重要因素。为了确定不同种族间唾液黏蛋白的浓度是否存在差异，我们测量了非裔美国人和白种人全唾液中黏液糖蛋白MG1和黏液糖蛋白MG2的浓度。方法：采集19名非裔美国人（4名男性，15名女性，平均年龄34岁，范围19 - 53岁）和25名高加索人（11名男性，14名女性，平均年龄31岁，范围20-51岁）志愿者在上午11点至中午12点咀嚼刺激（1 g Parafilm， 60次/分钟）下的全唾液。唾液总碳水化合物采用周期性酸希夫法测定，总蛋白采用215 nm吸光度法测定。采用免疫试剂对MG2进行酶联免疫吸附/酶联凝集素联合测定（ELISA/ELLA），对MG1进行捕获ELISA测定。结果：非洲裔美国人的总碳水化合物、蛋白质、MG1和MG2值分别为24.4±11.9、243.5±62.7、21.8±13.4和11.6±9.5 mg%，白种人的相应值分别为23.3±9.3、221.7±39.7、25.7±16.2和10.9±8.7 mg%。两组间测量的任何参数均无统计学差异。此外，研究表明，在非裔美国人和白种人中，唾液流速与碳水化合物、蛋白质或唾液粘蛋白的浓度之间不存在相关性。这些结果表明，流速对两组唾液参数的测量值没有影响。结论：在非裔美国人和白种人的全唾液中，唾液黏液蛋白MG1和MG2的浓度没有发现差异，黏液蛋白水平的变化似乎不太可能影响这些人群中胃食管反流的患病率。
OBJECTIVES:Organic and inorganic constituents of saliva have been implicated as protective components in the esophagus, and deficiencies in one or more of these factors in different races may be an important element in the prevalence of gastroesophageal reflux disease (GERD). To determine whether there are differences in the concentration of salivary mucins between different racial groups, we measured the concentration of mucous glycoprotein MG1 and mucous glycoprotein MG2 in whole saliva of African-Americans and Caucasians.METHODS:Whole saliva was collected from 19 African-American (four male, 15 female; mean age 34 yr, range 19–53 yr,) and 25 Caucasian (11 male, 14 female; mean age 31 yr, range 20–51 yr,) volunteers under masticatory stimulation (1 g Parafilm, 60 strokes/min) between 11: 00 AM and 12: 00 noon. Total salivary carbohydrate was measured with a periodic acid-Schiff assay and total protein by absorbance at 215 nm. Immunological reagents were employed to quantify MG2 in a combined enzyme-linked immunosorbent assay/enzyme linked lectin assay (ELISA/ELLA) and to quantify MG1 in a capture ELISA.RESULTS:The total carbohydrate, protein, MG1 and MG2 values were 24.4±11.9, 243.5±62.7, 21.8±13.4, and 11.6±9.5 mg% for African-Americans, and the corresponding values were 23.3±9.3, 221.7±39.7, 25.7±16.2, and 10.9±8.7 mg% for Caucasians. There was no statistical difference for any of the parameters measured between the two groups. Furthermore, it was shown that no correlation existed between salivary flow rate and the concentration of carbohydrate, protein, or salivary mucins in African-Americans and in Caucasians. These results show that flow rate did not influence the measured values for salivary parameters in the two groups.CONCLUSIONS:No differences were found in the concentration of salivary mucins MG1 and MG2 in whole saliva of African-Americans and Caucasians, and it seems unlikely that variations in mucin levels influence the prevalence of GERD in these groups.