GLP-1 IS REQUIRED IN THE GERM LINE FOR REGULATION OF THE DECISION BETWEEN MITOSIS AND MEIOSIS IN C-ELEGANS
GLP-1 IS REQUIRED IN THE GERM LINE FOR REGULATION OF THE DECISION BETWEEN MITOSIS AND MEIOSIS IN C-ELEGANS
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DOI：
10.1016/0092-8674(87)90128-0
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发表时间：
1987-11-20
期刊：
影响因子：
64.5
通讯作者：
KIMBLE, J
中科院分区：
文献类型：
--
作者：
AUSTIN, J;KIMBLE, J
在野生型C.线虫生殖细胞系中既有丝分裂的生殖细胞，也有减数分裂的生殖细胞。glp-1的突变导致正常情况下进行有丝分裂的生殖细胞进入减数分裂。这种突变体表型模拟了杀死远端细胞的作用，远端细胞是一种与生殖系相互作用以调节有丝分裂/减数分裂决定的体细胞。此外，野生型glp-1产物是胚胎发生所必需的母体产物。温度变化实验表明，在生殖细胞系中glp-1活性的时间要求与远端细胞调节的时间要求相同。镶嵌分析表明glp-1是在生殖细胞系中产生的。我们认为glp-1在远端细胞和生殖细胞之间的相互作用中是接受机制的一部分。
In the wild-type C. elegans germ line there are both mitotic and meiotic germ cells. Mutations in glp-1 cause germ cells that would normally divide mitotically to enter meiosis. This mutant phenotype mimics the effect of killing the distal tip cell, a somatic cell that interacts with the germ line to regulate the mitotic/meiotic decision. In addition, wild-type glp-1 product is required maternally for embryogenesis. Temperature-shift experiments indicate that the temporal requirement for glp-1 activity in the germ line is the same as that for distal tip cell regulation. Mosaic analyses suggest that glp-1 is produced in the germ line. We propose that glp-1 acts as part of the receiving mechanism in the interaction between the distal tip cell and germ line.