Multi-Functional Separation Mode-Ion Chromatography Using L-Pyroglutamic Acid Eluent for Simultaneous Determination of Sugars, Organic Acids, and Ethanol during Multiple Parallel Fermentation of Rice Wine
Multi-Functional Separation Mode-Ion Chromatography Using L-Pyroglutamic Acid Eluent for Simultaneous Determination of Sugars, Organic Acids, and Ethanol during Multiple Parallel Fermentation of Rice Wine
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DOI：
10.1080/03610470.2022.2158437
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发表时间：
2022-12-28
影响因子：
2
通讯作者：
Kozaki，Daisuke
中科院分区：
文献类型：
--
作者：
Takeoka，Marino;Hoki，Yoshiro;Kozaki，Daisuke
需要一种有效的评估方法来理解发酵，以支持新开发的酵母菌株在酿造中的实施。本课题组开发了一种多功能分离模式--离子色谱（MFS-IC），结合电导率和折射率检测器，用于分离关键组分（葡萄糖、乙醇和有机酸：丙酮酸、柠檬酸、L-苹果酸和琥珀酸）与乙基α-D-葡萄糖苷和甘油，以了解酿造过程中的发酵行为。采用强阳离子交换剂改性的小分子排阻色谱分离柱，以L-焦谷氨酸为洗脱剂，在45 min内获得了测定黄酒中8种关键物质的最佳分离度。了解黄酒发酵过程中黄酒样品中分析物的行为，可以监测糖化、糖酵解、乙醇发酵和三羧酸（TCA）循环的进展。这些结果有助于了解发酵特性，并在发酵领域实施新开发的酵母菌株。
An effective evaluation method for understanding fermentation is required to support the implementation of newly developed yeast strains in brewing. Our group developed a multi-functional separation mode-ion chromatography (MFS-IC) with conductivity and refractive index detectors to separate key species (glucose, ethanol, and organic acids: pyruvate, citrate, L-malate, and succinate) with ethylα-D-glucoside and glycerol to understand the fermentation behavior in brewing. Optimal chromatographic resolutions for determining eight different key species in rice wine by the MFS-IC were obtained in 45 min on low-molecular size-exclusion chromatographic separation columns modified with a strong cation-exchanger as the stationary phase with an eluent of L-pyroglutamic acid. Understanding the behavior of analytes in rice wine samples during rice wine fermentation enables the monitoring of the progress of saccharification, glycolysis, ethanol fermentation, and the tricarboxylic acid (TCA) cycle. These results are expected to contribute to understanding fermentation characteristics and implementing newly developed yeast strains in the fermentation field.