Association of the molecular regulation of ear leaf senescence/stress response and photosynthesis/metabolism with heterosis at the reproductive stage in maize.
Association of the molecular regulation of ear leaf senescence/stress response and photosynthesis/metabolism with heterosis at the reproductive stage in maize.
复制标题
玉米穗叶衰老/胁迫反应和光合作用/代谢的分子调控与繁殖期杂种优势的关系
DOI：
10.1038/srep29843
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发表时间：
2016-07-20
影响因子：
4.6
通讯作者：
Kuai B
中科院分区：
文献类型：
--
作者：
Song Y;Zhang Z;Tan X;Jiang Y;Gao J;Lin L;Wang Z;Ren J;Wang X;Qin L;Cheng W;Qi J;Kuai B
玉米表现出广泛的杂种优势性状，但杂种优势在生殖阶段的分子基础很少被利用。叶片衰老是一种影响作物产量和品质的退化过程。在本研究中，B73/Mo17和郑58/长7-2的互交杂交种在吐丝后穗叶衰老明显延迟，且所有杂交种的叶面积和茎长都较大，产量也较高。我们的时间序列转录组分析发现，两亲本系（PP-DEGs）之间存在2826个差异表达基因（DEGs），亲本系与杂种（PH-DEGs）之间存在2328个差异表达基因（DEGs）。值得注意的是，几个促进衰老的基因（ZmNYE1、ZmORE1、zmwrky53和zmpifs）在杂交后代中表现出低显性表达模式，而光合作用和碳固定（ZmPEPC）相关基因、淀粉生物合成基因（ZmAPS1、ZmAPL）、赤霉素生物合成基因（ZmGA20OX、ZmGA3OX）则表现出显性表达。我们还从PH-DEGs中鉴定出86个转录因子，其中一些已知可调节衰老、应激和代谢过程。总之，我们证明了穗叶衰老/胁迫响应和光合/代谢调控与发育后期杂种优势的分子关联。这一发现不仅扩大了我们对玉米杂种优势分子基础的认识，也为分子育种提供了基础信息。
Maize exhibits a wide range of heterotic traits, but the molecular basis of heterosis at the reproductive stage has seldom been exploited. Leaf senescence is a degenerative process which affects crop yield and quality. In this study, we observed significantly delayed ear leaf senescence in the reciprocal hybrids of B73/Mo17 and Zheng58/Chang7-2 after silking and all the hybrids displayed larger leaf areas and higher stems with higher yields. Our time-course transcriptome analysis identified 2,826 differentially expressed genes (DEGs) between two parental lines (PP-DEGs) and 2,328 DEGs between parental lines and the hybrid (PH-DEGs) after silking. Notably, several senescence promoting genes (ZmNYE1, ZmORE1, ZmWRKY53andZmPIFs) exhibited underdominant expression patterns in the hybrid, whereas putative photosynthesis and carbon-fixation (ZmPEPC)-associated, starch biosynthetic (ZmAPS1, ZmAPL), gibberellin biosynthetic genes (ZmGA20OX,ZmGA3OX) expressed overdominantly. We also identified 86 transcription factors from PH-DEGs, some of which were known to regulate senescence, stress and metabolic processes. Collectively, we demonstrate a molecular association of the regulations of both ear leaf senescence/stress response and photosynthesis/metabolism with heterosis at the late developmental stage. This finding not only extends our understanding to the molecular basis of maize heterosis but also provides basic information for molecular breeding.