Three-dimensional shape of the Golgi apparatus in different cell types: serial section scanning electron microscopy of the osmium-impregnated Golgi apparatus
Three-dimensional shape of the Golgi apparatus in different cell types: serial section scanning electron microscopy of the osmium-impregnated Golgi apparatus
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DOI：
10.1093/jmicro/dfv360
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发表时间：
2016-04-01
期刊：
影响因子：
1.8
通讯作者：
Ushiki, Tatsuo
中科院分区：
文献类型：
--
作者：
Koga, Daisuke;Kusumi, Satoshi;Ushiki, Tatsuo
尽管已经用光学和电子显微镜对高尔基体结构进行了许多研究，但由于先前应用的显微镜技术的技术限制，高尔基体的完整形状仍然不清楚。在本研究中，我们使用连续切片扫描电子显微镜(SEM)对高尔基体进行了形态研究。与聚焦离子束扫描(FIB-SEM)和连续块面扫描(SBF-SEM)不同，这种方法适用于细胞结构的三维(3D)重建，而不需要专门的仪器。用本实验室研制的连续切片扫描电子显微镜方法，观察了大鼠附睾腺泡细胞、胰腺腺泡细胞和促性腺激素细胞内高尔基体的三维形态。连续切片扫描和3D重建技术的结合使我们能够阐明这些细胞中高尔基体的完整形状。附睾腺细胞内高尔基体形态完整，呈篮状结构，呈椭圆形；胰腺腺泡细胞内高尔基体由细长的带状结构组成，彼此相连，形成粗大的网状结构。来自促性腺激素的高尔基体蓄水池的整体图像看起来像一个球形笼子。这项研究清楚地表明，高尔基体的整个3D形状随细胞类型的不同而不同，高尔基池网络看起来像一个位于细胞质大区域的单个肿块。
Although many studies of the Golgi apparatus structure have been performed by light and electron microscopy, the full shape of the Golgi apparatus remained unclear due to the technical limitations of the previously applied microscopy techniques. In this study, we used serial section scanning electron microscopy (SEM) for the morphological study of the Golgi apparatus. This method is useful for three-dimensional (3D) reconstruction of cellular structures without requiring specialized instruments, unlike focused ion beam SEM (FIB-SEM) and serial block face SEM (SBF-SEM). Using the serial section SEM method developed by our laboratory, we investigate the 3D shape of the osmium-impregnated Golgi apparatus in rat epididymal cells, pancreatic acinar cells and gonadotropes. The combination of serial section SEM and a 3D reconstruction technique enabled us to elucidate the entire shape of the Golgi apparatus in these cells. The full shape of the Golgi apparatus in epididymal cells formed a basket-like structure with oval-shaped cisterns, while the Golgi apparatus in an acinar cell from the pancreas was composed of elongated ribbon-like structures that were connected to each other, making a coarse network. The overall image of the Golgi apparatus cisterns from a gonadotrope looked like a spherical cage. This study has clearly shown that entire 3D shape of the Golgi apparatus varies depending on the cell type and that the Golgi cisterns network appears as a single mass located in the large region of the cytoplasm.