Methylparaben potentiates UV-induced damage of skin keratinocytes
Methylparaben potentiates UV-induced damage of skin keratinocytes
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DOI：
10.1016/j.tox.2006.07.018
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发表时间：
2006-10-03
期刊：
影响因子：
4.5
通讯作者：
Yoshikawa, Toshikazu
中科院分区：
文献类型：
--
作者：
Handa, Osamu;Kokura, Satoshi;Yoshikawa, Toshikazu
多年来，对羟基苯甲酸甲酯 (MP) 一直被用作化妆品中的防腐剂。在这项研究中，我们研究了紫外线 B (UVB) 暴露对 MP 处理的人体皮肤角质形成细胞的影响。 HaCaT角质形成细胞在含MP的培养基中培养24小时，暴露于UVB（15或30 mJ/cm(2)）并进一步培养24小时。随后的细胞活力通过基于 MTT 的测定进行量化，细胞死亡通过荧光显微镜和流式细胞术进行鉴定。使用荧光探针测量氧化应激、一氧化氮 (NO) 产生和细胞脂质过氧化。此外，通过电动凝胶迁移测定评估了核因子 kappa B 和激活蛋白 1 的激活。 MP 的实际浓度 (0.003%) 对 HaCaT 角质形成细胞中的细胞活力、氧化应激、NO 产生、脂质过氧化和核转录因子激活影响很小或没有影响。低剂量 UVB 对 HaCaT 角质形成细胞中的这些参数也几乎没有影响或没有影响。然而，UVB 暴露显着增加 MP 处理的 HaCaT 角质形成细胞中的细胞死亡、氧化应激、NO 产生、脂质过氧化和转录因子激活。这些结果表明，MP被认为是化妆品中安全的防腐剂，但暴露在阳光下可能会对人体皮肤产生有害影响。 (c) 2006 Elsevier Ireland Ltd. 保留所有权利。
For many years, methylparaben (MP) has been used as a preservative in cosmetics, In this study, we investigated the effects of ultraviolet-B (UVB) exposure on MP-treated human skin keratinocytes. HaCaT keratinocyte was cultured in MP-containing medium for 24 h, exposed to UVB (15 or 30 mJ/cm(2)) and further cultured for another 24 h. Subsequent cellular viability was quantified by MTT-based assay and cell death was qualified by fluorescent microscopy and flow cytometry. Oxidative stress, nitric oxide (NO) production and cellular lipid peroxidation were measured using fluorescent probes. In addition, activation of nuclear factor kappa B and activator protein-1 was assessed by electro-mobility gel-shift assay. Practical concentrations of MP (0.003%) had a little or no effect on cellular viability, oxidative stress, NO production, lipid peroxidation and activation of nuclear transcription factors in HaCaT keratinocytes. Low-dose UVB also had little or no effect on these parameters in HaCaT keratinocytes. However, UVB exposure significantly increased cell death, oxidative stress, NO production, lipid peroxidation and activation of transcription factors in MP-treated HaCaT keratinocytes. These results indicate that MP, which has been considered a safe preservative in cosmetics, may have harmful effects on human skin when exposed to sunlight. (c) 2006 Elsevier Ireland Ltd. All rights reserved.