Comparative evaluation of different calcium phosphate-based bone graft granules an in vitro study with osteoblast-like cells
Comparative evaluation of different calcium phosphate-based bone graft granules an in vitro study with osteoblast-like cells
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DOI：
10.1111/j.1600-0501.2011.02350.x
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发表时间：
2013-04-01
影响因子：
4.3
通讯作者：
Gelinsky, Michael
中科院分区：
文献类型：
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作者：
Bernhardt, Anne;Lode, Anja;Gelinsky, Michael
目的磷酸钙基颗粒状骨移植材料是牙种植领域骨再生的重要材料。两种新开发的骨移植材料是Ceracell（R），一种由磷酸三钙（-TCP）制成的开孔高度多孔生物陶瓷，添加了微晶玻璃和Osseolive（R），一种开孔多孔玻璃陶瓷，通式为Ca 2KNa（PO 4）2。本研究的目的是与已建立的陶瓷骨移植物Cerasorb M（R）、NanoBone（R）和BONIT Matrix（R）相比，在体外表征两种骨移植材料的不同改良。材料与方法通过DNA含量和乳酸脱氢酶（LDH）活性定量检测和扫描电镜（SEM）定性检测成骨样细胞SaOS-2的粘附和增殖情况。此外，应用MTT细胞活力染色以确认活细胞与不同材料的附着。成骨分化通过碱性磷酸酶（ALP）活性的测量以及成骨标志物的基因表达分析，使用逆转录酶PCR进行评估。结果Ceracell和Cerasorb M的DNA含量和LDH活性均显示出良好的细胞贴壁和增殖能力。当与细胞培养基预孵育时，Osseolive也显示出良好的细胞附着和增殖。成骨细胞样细胞在NanoBone和BONIT基质上的附着和增殖非常低，即使在与细胞培养基预孵育后也是如此。Ceracell（R）、Osseolive（R）和Cerasorb M（R）上的特异性ALP活性随时间增加，并且证实了骨相关基因ALP、骨粘连蛋白、骨桥蛋白和骨唾液蛋白II的表达。结论Ceracell和Osseolive颗粒在体外支持细胞增殖和成骨分化，可能是体内应用的有希望的候选物。
Objective Granule-shaped calcium phosphate-based bone graft materials are often required for bone regeneration especially in implant dentistry. Two newly developed bone graft materials are Ceracell (R), an open-celled highly porous bioceramic from -tricalcium phosphate (-TCP) under addition of bioglass and Osseolive (R), an open porous glass ceramic with the general formula Ca2KNa(PO4)2. The goal of this study was to characterize different modifications of the two bone graft materials in vitro in comparison to already established ceramic bone grafts Cerasorb M (R), NanoBone (R) and BONIT Matrix (R). Materials and methods Adhesion and proliferation of SaOS-2 osteoblast-like cells were evaluated quantitatively by determining DNA content and lactate dehydrogenase (LDH) activity and qualitatively by scanning electron microscopy (SEM). In addition, MTT cell-vitality staining was applied to confirm the attachment of viable cells to the different materials. Osteogenic differentiation was evaluated by measurement of alkaline phosphatase (ALP) activity as well as gene expression analysis of osteogenic markers using reverse transcriptase PCR. Results DNA content and LDH activity revealed good cell attachment and proliferation for Ceracell and Cerasorb M. When pre-incubated with cell-culture medium, also Osseolive showed good cell attachment and proliferation. Attachment and proliferation of osteoblast-like cells on NanoBone and BONIT Matrix was very low, even after pre-incubation with cell-culture medium. Specific ALP activity on Ceracell (R), Osseolive (R) and Cerasorb M (R) increased with time and expression of bone-related genes ALP, osteonectin, osteopontin and bone sialoprotein II was demonstrated. Conclusions Ceracell as well as Osseolive granules support proliferation and osteogenic differentiation in vitro and may be promising candidates for in vivo applications.